Rabbit PIK3C2A Polyclonal Antibody | anti-PIK3C2A antibody

PIK3C2A Polyclonal Antibody

Cat. Num. AAA9128036

• Gene Names: PIK3C2A; CPK; PI3-K-C2A; PI3-K-C2(ALPHA)
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: PIK3C2A; Polyclonal Antibody; PIK3C2A Polyclonal Antibody; anti-PIK3C2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 1686

• Applicable Applications for anti-PIK3C2A antibody: Western Blot (WB)
• Application Notes: WB 1:500 - 1:2000
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human PIK3C2A
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PIK3C2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse heart using PIK3C2A antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat heart using PIK3C2A antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using PIK3C2A antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-PIK3C2A antibody

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 157671929
• NCBI GeneID: 5286
• NCBI Accession #: NP_002636.2
• NCBI GenBank Nucleotide #: NM_002645.2
• UniProt Accession #: O00443; Q14CQ9; B0LPH2; B4E2G4
• Molecular Weight: 191kDa
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha
• NCBI Official Symbol: PIK3C2A
• NCBI Official Synonym Symbols: CPK; PI3-K-C2A; PI3-K-C2(ALPHA)
• NCBI Protein Information: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
• Protein Family: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing
• UniProt Gene Name: PIK3C2A
• UniProt Synonym Gene Names: PI3K-C2-alpha; PtdIns-3-kinase C2 subunit alpha
• UniProt Entry Name: P3C2A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

PIK3C2A: a protein of the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. Its PI3-kinase is not sensitive to nanomolar levels of the inhibitor wortmannin. Activated by insulin and may be involved in integrin-dependent signaling.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; Autophagy; EC 2.7.1.154

Chromosomal Location of Human Ortholog: 11p15.5-p14

Cellular Component: Golgi apparatus; membrane; clathrin-coated vesicle; cytoplasm; plasma membrane; cytosol; phosphoinositide 3-kinase complex; nucleus; vesicle

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; phosphoinositide binding; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; clathrin cage assembly; exocytosis; phosphoinositide-mediated signaling; phosphoinositide phosphorylation; phospholipid metabolic process; vascular smooth muscle contraction; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; endocytosis