Rabbit anti-Human PIGP Polyclonal Antibody | anti-PIGP antibody

Anti-PIGP Antibody, Rabbit Polyclonal

Cat. Num. AAA8109548

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PIGP; Polyclonal Antibody; Anti-PIGP Antibody; Rabbit Polyclonal; PIGP Antibody; Rabbit PAb; Antigen Affinity Purified; phosphatidylinositol glycan anchor biosynthesis; class P; Anti-DCRC Antibody; Anti-DCRC-S Antibody; Anti-DSCR5 Antibody; Anti-DSRC Antibody; anti-PIGP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PIGP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PIGP antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human PIGP fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PIGP fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PIGP rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: THP1 Whole Cell Lysate Lane C: U87MG Whole Cell Lysate Lane D: SHSY5Y Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa)

Immunoprecipitation (IP)

(PIGP was immunoprecipitated using: Lane A:0.5 mg U87MG Whole Cell Lysate 4 uL anti-PIGP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PIGP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size :20 kDa)

Related Product Information for anti-PIGP antibody

PIGP (Phosphatidylinositol Glycan Anchor Biosynthesis Class P) is a Protein Coding gene. The PIGP gene, located on 21q22.13, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, and frog. This gene encodes an enzyme involved in the first step of glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells that serve to anchor proteins to the cell surface. The encoded protein is a component of the GPI-N-acetylglucosaminyltransferase complex that catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). This gene is located in the Down Syndrome critical region on chromosome 21 and is a candidate for the pathogenesis of Down syndrome. This gene has multiple pseudogenes and is a member of the phosphatidylinositol glycan anchor biosynthesis gene family.

References

Shao M, et al. (2009) Down syndrome critical region protein 5 regulates membrane localization of wnt receptors, dishevelled stability and convergent extension in vertebrate embryos. Development 136 (12): 2121-2131.Johnstone DL, et al. (2017) Compound heterozygous mutations in the gene pigp are associated with early infantile epileptic encephalopathy. Hum Mol Genet 26 (9): 1706-1715.Ferrando-Miguel R, et al. (2004) Protein levels of genes encoded on chromosome 21 in fetal down syndrome brain (part v): Overexpression of phosphatidyl-inositol-glycan class p protein (dscr5). Amino Acids 26 (3): 255-261.