Western Blot (WB)
(Western Blot analysis of extracts from P3HR-1 and Ramos cells using MBS605724.)
anti-Mouse Activation-induced Cytidine Deaminase Monoclonal Antibody | anti-AICDA antibody
Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos)
Gene Names
AICDA; AID; ARP2; CDA2; HIGM2
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Purity
Ascites
Synonyms
Activation-induced Cytidine Deaminase; Monoclonal Antibody; Activation-induced Cytidine Deaminase (AICDA; AID; ARP2; CDA2; Cytidine Aminohydrolase; HIGM2; Integrated into Burkitt's Lymphoma Cell Line Ramos); Anti -Activation-induced Cytidine Deaminase (AICDA; anti-AICDA antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10g133
Specificity
Recognizes endogenous levels of human AID protein.
Purity/Purification
Ascites
Form/Format
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-AICDA antibody
Western Blot (WB), Immunoprecipitation (IP).
Other applications not tested.
Other applications not tested.
Application Notes
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic peptide corresponding to residues of human AID.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of extracts from P3HR-1 and Ramos cells using MBS605724.)
Western Blot (WB)
(Western Blot analysis of extracts from P3HR-1 and Ramos cells using MBS605724.)
Western Blot (WB)
(Western Blot analysis of immunoprecipitates from HeLa cell extracts overexpressing myc-tagged AID. Expression of myc-AID was confirmed (lane 1). Immunoprecipitations using cell lysates with or without expression of myc-AID were performed using MBS605724 (anti-AID antibody), M9601-30 (anti-Myc-Tag antibody), mouse IgG, or rabbit IgG. Immunoblot was performed using M9601-30 (anti-Myc-Tag antibody).)
Western Blot (WB)
(Western Blot analysis of immunoprecipitates from HeLa cell extracts overexpressing myc-tagged AID. Expression of myc-AID was confirmed (lane 1). Immunoprecipitations using cell lysates with or without expression of myc-AID were performed using MBS605724 (anti-AID antibody), M9601-30 (anti-Myc-Tag antibody), mouse IgG, or rabbit IgG. Immunoblot was performed using M9601-30 (anti-Myc-Tag antibody).)
Related Product Information for anti-AICDA antibody
Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation. The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function. AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma).
Product Categories/Family for anti-AICDA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
activation-induced cytidine deaminase
NCBI Official Synonym Full Names
activation-induced cytidine deaminase
NCBI Official Symbol
AICDA
NCBI Official Synonym Symbols
AID; ARP2; CDA2; HIGM2
NCBI Protein Information
activation-induced cytidine deaminase; OTTHUMP00000239182; OTTHUMP00000239185; OTTHUMP00000239186; cytidine aminohydrolase; integrated into Burkitt's lymphoma cell line Ramos
UniProt Protein Name
AID
Protein Family
UniProt Gene Name
AID
UniProt Synonym Gene Names
AICDA
UniProt Entry Name
Q546Y9_HUMAN
NCBI Description
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq]
Research Articles on AICDA
Similar Products
Product Notes
The AICDA aid (Catalog #AAA605724) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Activation-induced Cytidine Deaminase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Other applications not tested. Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the AICDA aid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Activation-induced Cytidine Deaminase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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