Rabbit PID Polyclonal Antibody | anti-MTA2 antibody

PID Antibody

Cat. Num. AAA151171

• Gene Names: MTA2; PID; MTA1L1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: PID Antibody is affinity chromatography purified via peptide column.
• Synonyms: PID; Polyclonal Antibody; PID Antibody; MTA1L1; Metastasis-associated protein MTA2; Metastasis-associated 1-like 1; MTA1-L1 protein; metastasis associated 1 family; member 2; anti-MTA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PID antibody is predicted to not cross-react with MTA2
• Purity/Purification: PID Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 668

• Applicable Applications for anti-MTA2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: PID antibody can be used for detection of PID by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PID antibody was raised against a 17 amino acid peptide near the carboxy terminus of human PID.
• Buffer: PID Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PID antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL. )

Immunocytochemistry (IHC)

(Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.)

Related Product Information for anti-MTA2 antibody

PID Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex. PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis. PID/MTA2 is ubiquitously expressed in human tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 9931638
• NCBI GeneID: 9219
• UniProt Accession #: O94776; Q68DB1; Q9UQB5
• Molecular Weight: Predicted: 73 kDa; ; Observed: 75 kDa
• NCBI Official Full Name: p53 target protein in deacetylase complex
• NCBI Official Synonym Full Names: metastasis associated 1 family, member 2
• NCBI Official Symbol: MTA2
• NCBI Official Synonym Symbols: PID; MTA1L1
• NCBI Protein Information: metastasis-associated protein MTA2; MTA1-L1 protein; metastasis-associated 1-like 1; metastasis-associated protein 2; metastasis -associated gene 1-like 1; p53 target protein in deacetylase complex; metastasis associated gene family, member 2
• UniProt Protein Name: Metastasis-associated protein MTA2
• Protein Family: Metastasis-associated protein
• UniProt Gene Name: MTA2
• UniProt Synonym Gene Names: MTA1L1; PID; MTA1-L1 protein
• UniProt Entry Name: MTA2_HUMAN

NCBI Description

This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis. [provided by RefSeq, May 2011]

Uniprot Description

MTA2: May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.

Protein type: Transcription factor; DNA-binding; Motility/polarity/chemotaxis; Deacetylase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q12-q13.1

Cellular Component: nucleoplasm; transcription factor complex; protein complex; membrane; histone deacetylase complex; nuclear chromatin; NuRD complex

Molecular Function: protein binding; nucleosomal DNA binding; zinc ion binding; histone deacetylase activity; transcription factor activity

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; DNA methylation; ATP-dependent chromatin remodeling; positive regulation of transcription from RNA polymerase II promoter; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter