Rabbit anti-Human Calreticulin Polyclonal Antibody | anti-CALR; CRT antibody

Calreticulin polyclonal antibody

Cat. Num. AAA565369

• Gene Names: CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A affinity purified.
• Synonyms: Calreticulin; Polyclonal Antibody; Calreticulin polyclonal antibody; anti-CALR; CRT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human calreticulin. Detects a band of ~55kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 417

• Applicable Applications for anti-CALR; CRT antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1,000, ECL); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~55kDa by Western blot.
• Immunogen: Recombinant human calreticulin.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Calreticulin pAb: Lane 1: MW marker, Lane 2: 50 ng Calreticulin, Lane 3: HeLa, Lane 4: 3T3, Lane 5: PC12.)

Related Product Information for anti-CALR; CRT antibody

The multifunctional, multi-compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins as well as a Ca2+-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein), Crt contains the ER-retrieval sequence, KDEL, and is the soluble paralog of the ER membrane protein Calnexin (Cnx). Crts three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P-domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N-glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression.

Product Categories/Family for anti-CALR; CRT antibody

Cell Signaling; Protein Synthesis & Folding; Oxidative Stress

NCBI and Uniprot Product Information

• NCBI GI #: 117501
• NCBI GeneID: 811
• UniProt Accession #: P27797; Q6IAT4; Q9UDG2
• Molecular Weight: 48,142 Da
• NCBI Official Full Name: Calreticulin
• NCBI Official Synonym Full Names: calreticulin
• NCBI Official Symbol: CALR
• NCBI Official Synonym Symbols: RO; CRT; SSA; cC1qR; HEL-S-99n
• NCBI Protein Information: calreticulin
• UniProt Protein Name: Calreticulin
• Protein Family: Calreticulin
• UniProt Gene Name: CALR
• UniProt Synonym Gene Names: CRTC; ERp60
• UniProt Entry Name: CALR_HUMAN

NCBI Description

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

Uniprot Description

Calreticulin: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57. Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Belongs to the calreticulin family.

Protein type: Secreted; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Calcium-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: acrosome; cell surface; cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum lumen; external side of plasma membrane; extracellular region; extracellular space; focal adhesion; Golgi apparatus; intracellular; membrane; nucleus; perinuclear region of cytoplasm; polysome; proteinaceous extracellular matrix; sarcoplasmic reticulum lumen; smooth endoplasmic reticulum

Molecular Function: androgen receptor binding; calcium ion binding; carbohydrate binding; chaperone binding; complement component C1q binding; DNA binding; glycoprotein binding; hormone binding; integrin binding; iron ion binding; mRNA binding; peptide binding; protein binding; ubiquitin protein ligase binding; unfolded protein binding; zinc ion binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cardiac muscle cell differentiation; cell cycle arrest; cellular calcium ion homeostasis; cellular protein metabolic process; cortical actin cytoskeleton organization and biogenesis; glucocorticoid receptor signaling pathway; negative regulation of neuron differentiation; negative regulation of retinoic acid receptor signaling pathway; negative regulation of steroid hormone receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of translation; peptide antigen assembly with MHC class I protein complex; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of phagocytosis; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein export from nucleus; protein folding; protein maturation via protein folding; protein stabilization; receptor-mediated endocytosis; regulation of apoptosis; regulation of meiosis; regulation of transcription, DNA-dependent; response to drug; response to estradiol stimulus; response to testosterone stimulus; sequestering of calcium ion; spermatogenesis; unfolded protein response

Disease: Myelofibrosis; Thrombocythemia 1