Testing Data
Fc gamma RIIIB/CD16b Active Protein | FCGR3B active protein
Human CellExp Fc gamma RIIIB / CD16b, human recombinant
Gene Names
FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
Applications
SDS-Page
Purity
>=98%
Synonyms
Fc gamma RIIIB/CD16b; Human CellExp Fc gamma RIIIB / CD16b; human recombinant; FCGR3B; CD16B; FCG3B; FCGR3; FCG3; IGFR3; FCGR3B active protein
Host
HEK293 cells
Purity/Purification
>=98%
Form/Format
Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized
Appearance: Lyophilized
Sequence Length
233
Applicable Applications for FCGR3B active protein
SDS-PAGE
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
Measured by its ability to bind human IgG with an estimated KD < 150 nM.
Results
Measured by its ability to bind human IgG with an estimated KD < 150 nM.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for FCGR3B active protein
Background: CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcgammaRIIIa (CD16a) and FcgammaRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner. CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B also known as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Product Categories/Family for FCGR3B active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 21.7 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 38-50 kDa due to glycosylation.
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-B
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
Protein Family
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
UniProt Entry Name
FCG3B_HUMAN
NCBI Description
The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]
Uniprot Description
Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Research Articles on FCGR3B
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Product Notes
The FCGR3B fcgr3b (Catalog #AAA844284) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fc gamma RIIIB/CD16b can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the FCGR3B fcgr3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fc gamma RIIIB/CD16b, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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