Western Blot (WB)
(Western blot analysis of P4086-01 to detect PIAS1 in HL-60 tissue lysate.)
Rabbit anti-Human PIAS1 Polyclonal Antibody | anti-PIAS1 antibody
PIAS1, CT (E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PIAS1; Polyclonal Antibody; CT (E3 SUMO-protein Ligase PIAS1; Protein Inhibitor of Activated STAT Protein 1; Gu Binding Protein; GBP; RNA Helicase II Binding Protein; DEAD/H Box-binding Protein 1; DDXBP1); Anti -PIAS1; anti-PIAS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human PIAS1. Species sequence homology: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Applicable Applications for anti-PIAS1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1-2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Dilution: Western Blot: 1-2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunogen
Synthetic peptide corresponding to 15aa from human PIAS1 at C-terminus.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of P4086-01 to detect PIAS1 in HL-60 tissue lysate.)
Western Blot (WB)
(Western blot analysis of P4086-01 to detect PIAS1 in HL-60 tissue lysate.)
Western Blot (WB)
(Western blot analysis of PIAS1 (arrow) using P4086-01 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PIAS1 (arrow) using P4086-01 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with P4086-01 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with P4086-01 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-PIAS1 antibody
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Product Categories/Family for anti-PIAS1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
PIAS1
NCBI Official Synonym Full Names
protein inhibitor of activated STAT, 1
NCBI Official Symbol
PIAS1
NCBI Protein Information
E3 SUMO-protein ligase PIAS1; SUMO E3 ligase
Protein Family
Similar Products
Product Notes
The PIAS1 (Catalog #AAA6008307) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIAS1, CT (E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIAS1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the PIAS1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIAS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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