Rabbit anti-Human PI3KR5 Polyclonal Antibody | anti-PI3KR5 antibody

PI3KR5, ID (PIK3R5, Phosphoinositide 3-kinase regulatory subunit 5, PI3-kinase p101 subunit, Phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K) (Azide free) (HRP)

Cat. Num. AAA6333754

• Gene Names: PIK3R5; p101; FOAP-2; P101-PI3K; F730038I15Rik
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PI3KR5; Polyclonal Antibody; ID (PIK3R5; Phosphoinositide 3-kinase regulatory subunit 5; PI3-kinase p101 subunit; Phosphatidylinositol 4; 5-bisphosphate 3-kinase regulatory subunit; Protein FOAP-2; PtdIns-3-kinase p101; p101-PI3K) (Azide free) (HRP); PIK3R5; p101-PI3K; anti-PI3KR5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PI3KR5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PI3KR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399~429 amino acids from the center region of human PI3KR5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PI3KR5 Antibody (Center) Pab in K562 cell line lysates (35ug/lane). PI3KR5(arrow) was detected using the purified polyclonal antibody.)

Western Blot (WB)

(Western blot analysis of PIK3R5 (arrow) using rabbit polyclonal PIK3R5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R5 gene (Lane 2).)

Related Product Information for anti-PI3KR5 antibody

PI3KR5 is a regulatory subunit of the PI3K gamma complex. This protein, which interacts with G beta gamma proteins, is a heterodimer of a catalytic subunit (PI3KCG/p120) and a regulatory (PI3KR5a/p101) subunit.

Product Categories/Family for anti-PI3KR5 antibody

Antibodies; Protein Kinases

References

Brock, C., et al., J. Cell Biol. 160(1):89-99 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 217330639
• NCBI GeneID: 23533
• NCBI Accession #: NP_001136105.1
• NCBI GenBank Nucleotide #: NM_001142633.2
• UniProt Accession #: Q8WYR1; Q5G936; Q5G938; Q5G939; Q8IZ23; Q9Y2Y2; B0LPH4; D3DTS3
• Molecular Weight: 97,348 Da
• NCBI Official Full Name: phosphoinositide 3-kinase regulatory subunit 5 isoform 1
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 5
• NCBI Official Symbol: PIK3R5
• NCBI Official Synonym Symbols: p101; FOAP-2; P101-PI3K; F730038I15Rik
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 5; protein FOAP-2; ptdIns-3-kinase p101; PI3-kinase p101 subunit; phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit; phosphatidylinositol-4,5-bisphosphate 3-kinase regulatory subunit
• UniProt Protein Name: Phosphoinositide 3-kinase regulatory subunit 5
• UniProt Gene Name: PIK3R5
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit 5
• UniProt Entry Name: PI3R5_HUMAN

NCBI Description

Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011]

Uniprot Description

PIK3R5: Regulatory subunit of the PI3K gamma complex. Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. Interacts with G beta gamma proteins. Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli. Greatly activated by G gamma proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; cytoplasm; cytosol; nucleus

Molecular Function: G-protein beta/gamma-subunit binding; 1-phosphatidylinositol-3-kinase regulator activity

Biological Process: positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; platelet activation; phosphoinositide 3-kinase cascade; phospholipid metabolic process; phosphatidylinositol biosynthetic process; blood coagulation; regulation of phosphoinositide 3-kinase activity

Disease: Ataxia-oculomotor Apraxia 3