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Dot Blot (DB) (Dot blot analysis of Phospho-PI3KC3-S164 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Rabbit anti-Human PI3KC3 Polyclonal Antibody | anti-PI3KC3 antibody

PI3KC3, Phosphorylated (Ser164) (Phosphatidylinositol 3-kinase Catalytic Subunit Type 3, PtdIns-3-kinase Type 3, PI3-kinase Type 3, PI3K Type 3, Phosphoinositide-3-kinase Class 3, Phosphatidylinositol 3-Kinase p100 Subunit, hVps34, VPS34) (Azide free) (HR

Gene Names
PIK3C3; VPS34; hVps34
Reactivity
Human
Applications
ELISA, Dot Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
PI3KC3; Polyclonal Antibody; Phosphorylated (Ser164) (Phosphatidylinositol 3-kinase Catalytic Subunit Type 3; PtdIns-3-kinase Type 3; PI3-kinase Type 3; PI3K Type 3; Phosphoinositide-3-kinase Class 3; Phosphatidylinositol 3-Kinase p100 Subunit; hVps34; VPS34) (Azide free) (HR; anti-PI3KC3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PI3KC3 when phosphorylated at Ser164.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-PI3KC3 antibody
ELISA (EIA), Dot Blot (DB)
Application Notes
ELISA: 1:1,000
Dot Blot: 1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser164 of human PI3KC3 (KLH).
Conjugate
HRP
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-PI3KC3-S164 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Dot Blot (DB) (Dot blot analysis of Phospho-PI3KC3-S164 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Product Categories/Family for anti-PI3KC3 antibody
References
1. Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003). 2. Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 91; 98 kDa

Observed: 98 kDa
NCBI Official Full Name
phosphatidylinositol 3-kinase catalytic subunit type 3
NCBI Official Synonym Full Names
phosphatidylinositol 3-kinase, catalytic subunit type 3
NCBI Official Symbol
PIK3C3
NCBI Official Synonym Symbols
VPS34; hVps34
NCBI Protein Information
phosphatidylinositol 3-kinase catalytic subunit type 3; PI3K type 3; PI3-kinase type 3; PtdIns-3-kinase type 3; phosphoinositide-3-kinase, class 3; phosphatidylinositol 3-kinase p100 subunit
UniProt Protein Name
Phosphatidylinositol 3-kinase catalytic subunit type 3
UniProt Gene Name
PIK3C3
UniProt Synonym Gene Names
VPS34; PI3-kinase type 3; PI3K type 3
UniProt Entry Name
PK3C3_HUMAN

Research Articles on PI3KC3

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Product Notes

The PI3KC3 pik3c3 (Catalog #AAA6493225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI3KC3, Phosphorylated (Ser164) (Phosphatidylinositol 3-kinase Catalytic Subunit Type 3, PtdIns-3-kinase Type 3, PI3-kinase Type 3, PI3K Type 3, Phosphoinositide-3-kinase Class 3, Phosphatidylinositol 3-Kinase p100 Subunit, hVps34, VPS34) (Azide free) (HR reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PI3KC3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot Blot (DB). ELISA: 1:1,000 Dot Blot: 1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PI3KC3 pik3c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3KC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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