Rabbit anti-Human PI3CKG Polyclonal Antibody

PI3CKG, CT (Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit gamma Isoform, PtdIns-3-kinase Subunit gamma, PI3K-gamma, Phosphatidylinositol-4,5-bisphosphate 3-kinase 110kD Catalytic Subunit gamma, PtdIns-3-kinase Subunit p110-gamma, p120-P

Cat. Num. AAA629094

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PI3CKG; Polyclonal Antibody; CT (Phosphatidylinositol-4; 5-bisphosphate 3-kinase Catalytic Subunit gamma Isoform; PtdIns-3-kinase Subunit gamma; PI3K-gamma; Phosphatidylinositol-4; 5-bisphosphate 3-kinase 110kD Catalytic Subunit gamma; PtdIns-3-kinase Subunit p110-gamma; p120-P; Anti -PI3CKG; anti-PI3CKG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PI3CKG at ~126kD.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PI3CKG antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:50-1:100; Immunohistochemistry (FFPE): 1:10-1:50
• Immunogen: Synthetic peptide corresponding to a region near aa1048-1078 at the C-terminal of human PI3CKG, conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PI3CKG Antibody (C-term) Pab in Hela cell line lysates (35ug/lane). PI3CKG (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-PIK3CG Antibody (C-term) Pab in 293 cell line lysates transiently transfected with the PIK3CG gene (2ug/lane). PIK3CG(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human muscle tissue tissue reacted with PI3CKG antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PI3CKG antibody

This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.

Product Categories/Family for anti-PI3CKG antibody

Antibodies; Abs to Protein Kinases