Rabbit anti-Human, Mouse PHF21A Polyclonal Antibody | anti-PHF21A antibody

PHF21A Antibody

Cat. Num. AAA7052249

• Gene Names: PHF21A; BHC80; BM-006
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: PHF21A; Polyclonal Antibody; PHF21A Antibody; PHD finger protein 21A; BHC80a; BRAF35-HDAC complex protein BHC80; BHC80; KIAA1696; BM-006; anti-PHF21A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 680

• Applicable Applications for anti-PHF21A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human PHD finger protein 21A protein (312-487AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse liver tissue,Mouse kidney tissueAll lanes: PHF21A antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 75,71 KDaObserved band size: 75 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7052249 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7052249 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7052249 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-PHF21A antibody

Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.

NCBI and Uniprot Product Information

• NCBI GI #: 156546894
• NCBI GeneID: 51317
• NCBI Accession #: NP_001095272.1
• NCBI GenBank Nucleotide #: NM_001101802.1
• UniProt Accession #: Q96BD5; Q6AWA2; Q9C0G7; Q9H8V9; Q9HAK6; Q9NZE9; D3DQP5
• Molecular Weight: 74,982 Da
• NCBI Official Full Name: PHD finger protein 21A isoform a
• NCBI Official Synonym Full Names: PHD finger protein 21A
• NCBI Official Symbol: PHF21A
• NCBI Official Synonym Symbols: BHC80; BM-006
• NCBI Protein Information: PHD finger protein 21A
• UniProt Protein Name: PHD finger protein 21A
• Protein Family: PHD finger protein
• UniProt Gene Name: PHF21A
• UniProt Synonym Gene Names: BHC80; KIAA1696

NCBI Description

The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).[supplied by OMIM, Nov 2010]

Uniprot Description

PHF21A: Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: histone deacetylase complex; nucleoplasm

Molecular Function: chromatin binding; histone binding; histone deacetylase activity; protein binding; transcription cofactor activity

Biological Process: blood coagulation