Western Blot (WB)
(Western blot analysis of Rat Liver cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.)
Rabbit anti-Human; Rat Beclin 2 Polyclonal Antibody | anti-BECN2 antibody
Beclin 2 Antibody: Alkaline Phosphatase
Gene Names
BECN2; BECN1L1; BECN1P1
Reactivity
Human; Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Peptide Affinity Purified
Synonyms
Beclin 2; Polyclonal Antibody; Beclin 2 Antibody: Alkaline Phosphatase; Rabbit Anti-Human Beclin 2 Polyclonal; BECN1P1 Antibody; Beclin-1 autophagy-related pseudogene 1 Antibody; BECN2 Antibody; Beclin-2 Antibody; BECN1L1 Antibody; Beclin-1-like protein 1 Antibody; anti-BECN2 antibody
Host
Rabbit
Reactivity
Human; Rat
Clonality
Polyclonal
Specificity
Detects ~48 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
431
Applicable Applications for anti-BECN2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000
ICC/IF: 1:100
ICC/IF: 1:100
Target
Beclin 2
Immunogen
Synthetic peptide from the C-terminal of Human Beclin 2 (aa. 338-349)
Immunogen Species
Human
Cellular Localization
Cytoplasm
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Present in fetal and adult brain (at protein level).
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Rat Liver cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.)
Western Blot (WB)
(Western blot analysis of Rat Liver cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.)
Related Product Information for anti-BECN2 antibody
Beclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy. It has alson been found to mediate G protein-coupled receptro (GPCR) degradation (1).
Product Categories/Family for anti-BECN2 antibody
References
1. He C., et al. (2013) Cell. 154(5): 1085-1099.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,153 Da
NCBI Official Full Name
beclin-2
NCBI Official Synonym Full Names
beclin 2
NCBI Official Symbol
BECN2
NCBI Official Synonym Symbols
BECN1L1; BECN1P1
NCBI Protein Information
beclin-2
UniProt Protein Name
Beclin-2
Protein Family
UniProt Gene Name
BECN2
Uniprot Description
Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.
Research Articles on BECN2
Similar Products
Product Notes
The BECN2 becn2 (Catalog #AAA809391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Beclin 2 Antibody: Alkaline Phosphatase reacts with Human; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Beclin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ICC/IF: 1:100. Researchers should empirically determine the suitability of the BECN2 becn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beclin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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