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Western Blot (WB) (Western blot analysis of extracts of various celllines,using PI3 antibody. )

Rabbit anti-Human PI3 Polyclonal Antibody | anti-PI3 antibody

PI3 Polyclonal Antibody

Gene Names
PI3; ESI; WAP3; SKALP; WFDC14; cementoin
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
PI3; Polyclonal Antibody; PI3 Polyclonal Antibody; Cementoin; Elafin; Elastase specific inhibitor; ESI; Peptidase inhibitor 3; PI 3; Protease inhibitor WAP3; SKALP; WAP3; WFDC14; anti-PI3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Sequence Length
117
Applicable Applications for anti-PI3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
Immunogen
Recombinant protein of human PI3
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19756 / sc-20637
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various celllines,using PI3 antibody. )

Western Blot (WB) (Western blot analysis of extracts of various celllines,using PI3 antibody. )

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon using PI3 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon using PI3 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using PI3 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse lung using PI3 antibody at dilution of 1:100 (40x lens).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
12kDa
NCBI Official Full Name
PI3, partial
NCBI Official Synonym Full Names
peptidase inhibitor 3
NCBI Official Symbol
PI3
NCBI Official Synonym Symbols
ESI; WAP3; SKALP; WFDC14; cementoin
NCBI Protein Information
elafin
UniProt Protein Name
Elafin
Protein Family
UniProt Gene Name
PI3
UniProt Synonym Gene Names
WAP3; WFDC14; ESI; PI-3; SKALP
UniProt Entry Name
ELAF_HUMAN

NCBI Description

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014]

Uniprot Description

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Protein type: Extracellular matrix; Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix

Molecular Function: endopeptidase inhibitor activity

Research Articles on PI3

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Product Notes

The PI3 pi3 (Catalog #AAA2541358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI3 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PI3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:100 - 1:200. Researchers should empirically determine the suitability of the PI3 pi3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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