Western Blot (WB)
(PI15 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).)
Rabbit anti-Human PI15 Polyclonal Antibody
PI15, NT (PI15, CRISP8, P25TI, Peptidase inhibitor 15, 25kD trypsin inhibitor, Cysteine-rich secretory protein 8, SugarCrisp)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
PI15; Polyclonal Antibody; NT (PI15; CRISP8; P25TI; Peptidase inhibitor 15; 25kD trypsin inhibitor; Cysteine-rich secretory protein 8; SugarCrisp); Anti -PI15; anti-PI15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-PI15 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunogen
PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-63 amino acids from the N-terminal region of human PI15.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PI15 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).)
Western Blot (WB)
(PI15 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).)
Immunohistochemistry (IHC)
(PI15 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PI15 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PI15 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PI15 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PI15 antibody
This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq].
Product Categories/Family for anti-PI15 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PI15 (Catalog #AAA645279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI15, NT (PI15, CRISP8, P25TI, Peptidase inhibitor 15, 25kD trypsin inhibitor, Cysteine-rich secretory protein 8, SugarCrisp) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PI15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the PI15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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