Human Dynamin-2 ELISA Kit | DNM2 elisa kit

Human Dynamin-2 ELISA Kit

Cat. Num. AAA763581

• Gene Names: DNM2; DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB
• Reactivity: Human
• Synonyms: Dynamin-2; Human Dynamin-2 ELISA Kit; DNM2/Dynamin-2; DNM2 elisa kit

• Reactivity: Human
• Sequence Length: 866

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DNM2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Dynamin-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Dynamin-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Dynamin-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Dynamin-2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 56549125
• NCBI GeneID: 1785
• NCBI Accession #: NP_001005362.1
• NCBI GenBank Nucleotide #: NM_001005362.2
• Molecular Weight: 97,977 Da
• NCBI Official Full Name: dynamin-2 isoform 4
• NCBI Official Synonym Full Names: dynamin 2
• NCBI Official Symbol: DNM2
• NCBI Official Synonym Symbols: DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB
• NCBI Protein Information: dynamin-2
• UniProt Protein Name: Dynamin-2
• Protein Family: Dynamin
• UniProt Gene Name: DNM2
• UniProt Synonym Gene Names: DYN2
• UniProt Entry Name: DYN2_HUMAN

NCBI Description

Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq, Jun 2010]

Uniprot Description

DYN2: a cytoplasmic microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. May mediate the conventional clathrin-mediated uptake of surface receptors. Expressed in the postsynaptic density of neuronal cells. Two alternatively spliced isoforms have been described.

Protein type: EC 3.6.5.5; Microtubule-binding; Motility/polarity/chemotaxis; Vesicle; Hydrolase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: centrosome; clathrin-coated endocytic vesicle; coated pit; cytoplasm; cytosol; endosome; focal adhesion; Golgi apparatus; Golgi membrane; growth cone; lamellipodium; microtubule; midbody; nucleus; perinuclear region of cytoplasm; phagocytic cup; photoreceptor inner segment; plasma membrane; postsynaptic density; postsynaptic membrane; protein complex; trans-Golgi network

Molecular Function: D2 dopamine receptor binding; enzyme binding; GTP binding; GTPase activity; microtubule binding; nitric-oxide synthase binding; protein binding; protein kinase binding; SH3 domain binding; WW domain binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; endocytosis; G-protein coupled receptor internalization; G2/M transition of mitotic cell cycle; Golgi to plasma membrane transport; negative regulation of transforming growth factor beta receptor signaling pathway; neurite morphogenesis; nitric oxide metabolic process; positive regulation of apoptosis; positive regulation of nitric oxide biosynthetic process; positive regulation of phagocytosis; positive regulation of transcription, DNA-dependent; post-Golgi vesicle-mediated transport; receptor internalization; receptor-mediated endocytosis; regulation of axon extension; regulation of nitric-oxide synthase activity; regulation of Rac protein signal transduction; regulation of transcription, DNA-dependent; response to cocaine; response to light stimulus; signal transduction; spermatogenesis; synaptic vesicle transport; transferrin transport

Disease: Charcot-marie-tooth Disease, Dominant Intermediate B; Lethal Congenital Contracture Syndrome 5; Myopathy, Centronuclear, 1