Western Blot (WB)
(PI15 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).)
Rabbit anti-Human PI15 Polyclonal Antibody | anti-PI15 antibody
PI15 Antibody (N-term)
Western Blot (WB)
(PI15 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).)
Western Blot (WB)
(PI15 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).)
Immunohistochemistry (IHC)
(PI15 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PI15 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PI15 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PI15 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
similarity to insect venom allergens, mammalian testis-specific
proteins and plant pathogenesis-related proteins. It is frequently
expressed in human neuroblastoma and glioblastoma cell lines, and
thus may play a role in the central nervous system. [provided by
RefSeq].
Kaplan, F., et al. Am. J. Physiol. 276 (6 PT 1), L1027-L1036 (1999) :
Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)
Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)
Koshikawa, N., et al. J. Biochem. 119(2):334-339(1996)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq, Jul 2008]
Uniprot Description
PI15: Serine protease inhibitor which displays weak inhibitory activity against trypsin. Belongs to the CRISP family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8q21.11
Molecular Function: protease inhibitor activity
Biological Process: negative regulation of peptidase activity
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Product Notes
The PI15 pi15 (Catalog #AAA9210303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 34-63. The PI15 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PI15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PI15 pi15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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