Rabbit anti-Human PHPT1 Polyclonal Antibody | anti-PHPT1 antibody

PHPT1, NT (14kD Phosphohistidine Phosphatase, Phosphohistidine Phosphatase 1, Protein Janus-A Homolog, PHPT1, PHP14, CGI-202, HSPC141) (Azide free) (HRP)

Cat. Num. AAA6493304

• Gene Names: PHPT1; PHP14; CGI-202; HSPC141; HEL-S-132P
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PHPT1; Polyclonal Antibody; NT (14kD Phosphohistidine Phosphatase; Phosphohistidine Phosphatase 1; Protein Janus-A Homolog; PHP14; CGI-202; HSPC141) (Azide free) (HRP); anti-PHPT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHPT1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PHPT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PHPT1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PHPT1 (arrow) using rabbit polyclonal PHPT1 Antibody (Human N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PHPT1 antibody

Antibodies; Enzymes; Phosphatase

References

1. Lim, J., Cell 125(4), 801-814 (2006). 2. Mehrle, A., Nucleic Acids Res. 34, D415-D418 (2006). 3. Ma, R., Biochem. Biophys. Res. Commun. 337(3), 887-891 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 25008934
• NCBI GeneID: 29085
• NCBI Accession #: Q9NRX4.1
• NCBI GenBank Nucleotide #: NM_014172
• UniProt Accession #: Q9NRX4; Q9H0Y3; B1AMX0; B1AMX1
• Molecular Weight: 125
• NCBI Official Full Name: 14 kDa phosphohistidine phosphatase
• NCBI Official Synonym Full Names: phosphohistidine phosphatase 1
• NCBI Official Symbol: PHPT1
• NCBI Official Synonym Symbols: PHP14; CGI-202; HSPC141; HEL-S-132P
• NCBI Protein Information: 14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P
• UniProt Protein Name: 14 kDa phosphohistidine phosphatase
• Protein Family: 14 kDa phosphohistidine phosphatase
• UniProt Gene Name: PHPT1
• UniProt Synonym Gene Names: PHP14
• UniProt Entry Name: PHP14_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation