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Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: 293T) using a 5 % SDS PAGE gel and Phospholipase C eta 1 antibody at a dilution of 1:500)

Rabbit Phospholipase C eta 1 Polyclonal Antibody | anti-PLCH1 antibody

Phospholipase C eta 1 antibody

Gene Names
PLCH1; PLCL3
Applications
Western Blot
Purity
Phospholipase C eta 1 antibody was purified by antigen-affinity chromatography
Synonyms
Phospholipase C eta 1; Polyclonal Antibody; Phospholipase C eta 1 antibody; Polyclonal Phospholipase C eta 1; Anti-Phospholipase C eta 1; phospholipase C-eta-1; PLC-L3; Phospholipase C eta -1; PLC-eta-1; PLCH1; phospholipase C-eta1a; DKFZp434C1372; phospholipase C-like 3; PLCL3; phosphoinositide phospholipase C-eta-1; MGC117152; anti-PLCH1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Phospholipase C eta 1 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1655
Applicable Applications for anti-PLCH1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:3000
Biological Significance
PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate P2) to generate second messengers inositol 1,4,5-trisphosphate and diacylglycerol.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: 293T) using a 5 % SDS PAGE gel and Phospholipase C eta 1 antibody at a dilution of 1:500)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: 293T) using a 5 % SDS PAGE gel and Phospholipase C eta 1 antibody at a dilution of 1:500)
Related Product Information for anti-PLCH1 antibody
Affinity purified Rabbit polyclonal Phospholipase C eta 1 antibody
Product Categories/Family for anti-PLCH1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
185 kDa
NCBI Official Full Name
Phospholipase C, eta 1
NCBI Official Synonym Full Names
phospholipase C, eta 1
NCBI Official Symbol
PLCH1
NCBI Official Synonym Symbols
PLCL3
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
UniProt Gene Name
PLCH1
UniProt Synonym Gene Names
KIAA1069; PLCL3; PLC-eta-1; PLC-L3
UniProt Entry Name
PLCH1_HUMAN

NCBI Description

PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009]

Uniprot Description

PLCH1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.11; Phospholipase

Chromosomal Location of Human Ortholog: 3q25.31

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; cytosol

Molecular Function: signal transducer activity; calcium-dependent phospholipase C activity; calcium ion binding; phosphoinositide phospholipase C activity

Biological Process: inositol phosphate metabolic process; phosphoinositide-mediated signaling; lipid catabolic process

Research Articles on PLCH1

Similar Products

Product Notes

The PLCH1 plch1 (Catalog #AAA835074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phospholipase C eta 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:3000. Researchers should empirically determine the suitability of the PLCH1 plch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase C eta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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