Western Blot (WB)
(Western blot analysis of extracts of Jurkat using NUDT6 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit NUDT6 Polyclonal Antibody | anti-NUDT6 antibody
NUDT6 Antibody
Gene Names
NUDT6; GFG1; GFG-1; ASFGF2; FGF-AS; FGF2AS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NUDT6; Polyclonal Antibody; NUDT6 Antibody; Antisense basic fibroblast growth factor; FGF2AS; GFG1; Nucleoside diphosphate linked moiety X motif 6; Nucleoside diphosphate-linked moiety X motif 6; Nudix motif 6; NUDT6_HUMAN; Protein GFG; anti-NUDT6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NUDT6 antibody detects endogenous levels of total NUDT6
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for anti-NUDT6 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NUDT6
Subcellular Location
Nucleus; Mitochondrion
Tissue Specificity
Detected in liver, kidney and esophagus (at protein level). Ubiquitous.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Zebrafish (83%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of Jurkat using NUDT6 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of Jurkat using NUDT6 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9606725 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606725 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NUDT6 antibody
Description: NUDT6 (nudix (nucleoside diphosphate linked moiety X)-type motif 6), also known as Antisense basic fibroblast growth factor or GFG-1, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. The gene encoding NUDT6 is a FGF-2 gene antisense transcript, and NUDT6 is believed to regulate FGF-2 expression. FGF-2 is a mutifunctional heparin-binding growth factor important to angiogenesis, neuroectoderm development and wound healing. NUDT6 is expressed as two isoforms produced by alternative splicing.
Function: May contribute to the regulation of cell proliferation.
Subunit Structure: Monomer and homodimer.
Similarity: Belongs to the Nudix hydrolase family.
Function: May contribute to the regulation of cell proliferation.
Subunit Structure: Monomer and homodimer.
Similarity: Belongs to the Nudix hydrolase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 36 kDa
Predicted: 36 kDa
Predicted: 36 kDa
NCBI Official Full Name
nucleoside diphosphate-linked moiety X motif 6 isoform a
NCBI Official Synonym Full Names
nudix hydrolase 6
NCBI Official Symbol
NUDT6
NCBI Official Synonym Symbols
GFG1; GFG-1; ASFGF2; FGF-AS; FGF2AS
NCBI Protein Information
nucleoside diphosphate-linked moiety X motif 6
UniProt Protein Name
Nucleoside diphosphate-linked moiety X motif 6
Protein Family
UniProt Gene Name
NUDT6
UniProt Synonym Gene Names
FGF2AS; Nudix motif 6
NCBI Description
This gene overlaps and lies on the opposite strand from FGF2 gene, and is thought to be the FGF2 antisense gene. The two genes are independently transcribed, and their expression shows an inverse relationship, suggesting that this antisense transcript may regulate FGF2 expression. This gene has also been shown to have hormone-regulatory and antiproliferative actions in the pituitary that are independent of FGF2 expression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
May contribute to the regulation of cell proliferation.
Research Articles on NUDT6
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Product Notes
The NUDT6 nudt6 (Catalog #AAA9606725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUDT6 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NUDT6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NUDT6 nudt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDT6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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