Goat anti-Human Phospholipase A2, Secretory, Group IB Polyclonal Antibody | anti-PLA2G1B antibody

Phospholipase A2, Secretory, Group IB (PLA2G1B, phospholipase A2, MGC119834, MGC119835, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 precursor, PLA2, PLA2A, PPLA2)

Cat. Num. AAA624865

• Gene Names: PLA2G1B; PLA2; PLA2A; PPLA2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: Phospholipase A2; Secretory; Group IB; Polyclonal Antibody; Group IB (PLA2G1B; phospholipase A2; MGC119834; MGC119835; Phosphatidylcholine 2-acylhydrolase; Phospholipase A2 precursor; PLA2; PLA2A; PPLA2); Anti -Phospholipase A2; anti-PLA2G1B antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Phospholipase A2, Secretory, Group IB.
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PLA2G1B antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA Titer: 1:64,000; Western Blot: 0.01ug/ml; ~17-18kD band observed in Human Pancrease lysates (calcuated MW: 16.4kD according to NP_000919.1). In transfected HEK293 transiently expressing PLAG1B a band of ~18kD is observed. This band is not observed in the non-transfected HEK293.
• Immunogen: Synthetic peptide corresponding to NKAHKNLDTKKYCQS, from the C-Terminus of human Phospholipase A2, Secretory, Group IB (NP_000919.1).
• Species Sequence Homology: Canine.
• Positive Control: Human Pancreas lysates
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS624865 (0.01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Testing Data

(HEK293 overexpressing PLA2G1B and probed with MBS624865 (mock transfection in first lane).)

Related Product Information for anti-PLA2G1B antibody

Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentration dependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.

Product Categories/Family for anti-PLA2G1B antibody

Antibodies; Abs to Enzymes; Phospholipase

NCBI and Uniprot Product Information

• NCBI GI #: 4505847
• NCBI GeneID: 5319
• NCBI Accession #: NP_000919.1
• NCBI GenBank Nucleotide #: NM_000928.2
• UniProt Accession #: P04054; Q3KPI1; B2R4H5
• Molecular Weight: 16,360 Da
• NCBI Official Full Name: phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IB (pancreas)
• NCBI Official Symbol: PLA2G1B
• NCBI Official Synonym Symbols: PLA2; PLA2A; PPLA2
• NCBI Protein Information: phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B
• UniProt Protein Name: Phospholipase A2
• Protein Family: Phospholipase
• UniProt Gene Name: PLA2G1B
• UniProt Synonym Gene Names: PLA2; PLA2A; PPLA2
• UniProt Entry Name: PA21B_HUMAN

NCBI Description

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

Uniprot Description

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Secreted, signal peptide; Lipid Metabolism - alpha-linolenic acid; Secreted; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Phospholipase; DNA replication; Lipid Metabolism - linoleic acid; EC 3.1.1.4

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space; cell surface; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; bile acid binding; calcium ion binding; receptor binding

Biological Process: positive regulation of immune response; activation of MAPK activity; activation of phospholipase A2; neutrophil mediated immunity; glucose transport; signal transduction; activation of NF-kappaB transcription factor; phosphatidylcholine metabolic process; positive regulation of fibroblast proliferation; phospholipid metabolic process; fatty acid biosynthetic process; neutrophil chemotaxis; multicellular organismal lipid catabolic process; glycerophospholipid biosynthetic process; actin filament organization; arachidonic acid secretion; leukotriene biosynthetic process; defense response to Gram-positive bacterium; cellular response to insulin stimulus; positive regulation of protein secretion; innate immune response in mucosa; antibacterial humoral response; phosphatidic acid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA replication; interleukin-8 production