Rabbit Phospholamban Polyclonal Antibody | anti-PLN antibody

Anti-Phospholamban antibody

Cat. Num. AAA175456

• Gene Names: PLN; PLB; CMD1P; CMH18
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: Phospholamban; Polyclonal Antibody; Anti-Phospholamban antibody; Cardiac phospholamban(PLB); phospholamban; Cardiac phospholamban antibody; CMD1P antibody; CMH18 antibody; PLB antibody; PLN antibody; PPLA_HUMAN antibody; anti-PLN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 52

• Applicable Applications for anti-PLN antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Phospholamban (2-16aa EKVQYLTRSAIRRAS), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-PLN antibody, MBS175456, Western blottingAll lanes: Anti PLN (MBS175456) at 0.5ug/mlLane 1: Rat Heart Tissue Lysate at 50ugLane 2: Rat Heart Tissue Lysate at 50ugLane 3: CEM Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: HT1080 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugPredicted bind size: 18KDObserved bind size: 18KD )

Related Product Information for anti-PLN antibody

Description: Rabbit IgG polyclonal antibody for Cardiac phospholamban(PLN) detection. Tested with WB in Human, Mouse, Rat.
Background: Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. McTiernan et al.(1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.

NCBI and Uniprot Product Information

• NCBI GI #: 130774
• NCBI GeneID: 5350
• UniProt Accession #: P26678
• Molecular Weight: 6,109 Da
• NCBI Official Full Name: Cardiac phospholamban
• NCBI Official Synonym Full Names: phospholamban
• NCBI Official Symbol: PLN
• NCBI Official Synonym Symbols: PLB; CMD1P; CMH18
• NCBI Protein Information: cardiac phospholamban
• UniProt Protein Name: Cardiac phospholamban
• Protein Family: Cardiac phospholamban
• UniProt Gene Name: PLN
• UniProt Synonym Gene Names: PLB; PLB
• UniProt Entry Name: PPLA_HUMAN

NCBI Description

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]

Uniprot Description

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Membrane protein, integral; Inhibitor

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: sarcoplasmic reticulum membrane; membrane; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; vesicle

Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding

Biological Process: cardiac muscle development; negative regulation of calcium ion transport; negative regulation of heart rate; cytosolic calcium ion homeostasis; blood circulation; regulation of calcium ion transport; response to testosterone stimulus; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; protein homooligomerization; regulation of the force of heart contraction

Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18