Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue sample,using Phospholamban Antibody.)
Rabbit Phospholamban Polyclonal Antibody | anti-PLB antibody
Phospholamban Antibody
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue sample,using Phospholamban Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue sample,using Phospholamban Antibody.)
Post Translational Modifications: Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. Palmitoylated by ZDHHC16, promoting formation of the homopentamer.
Subunit Structure: Homopentamer (PubMed:16043693, PubMed:16897780). Interacts with HAX1 and ATP2A2 (PubMed:17241641).
Similarity: Belongs to the phospholamban family.
NCBI and Uniprot Product Information
Predicted: 7 kDa
NCBI Description
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016]
Uniprot Description
PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.
Protein type: Membrane protein, integral; Inhibitor
Chromosomal Location of Human Ortholog: 6q22.1
Cellular Component: sarcoplasmic reticulum membrane; mitochondrion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; vesicle
Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding
Biological Process: cardiac muscle development; negative regulation of calcium ion transport; negative regulation of heart rate; cytosolic calcium ion homeostasis; blood circulation; regulation of calcium ion transport; response to testosterone stimulus; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; regulation of the force of heart contraction; protein homooligomerization
Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18
Research Articles on PLB
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Product Notes
The PLB pln (Catalog #AAA9611066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospholamban Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholamban can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the PLB pln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholamban, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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