Rabbit anti-Human, Mouse Phosphoinositide 3 Kinase Enhancer Polyclonal Antibody | anti-LOC100557420 antibody

Phosphoinositide 3 Kinase Enhancer (PIKE, Centaurin gamma 1)

Cat. Num. AAA617095

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Highly Purified; Purified by Ion Exchange Chromatography.
• Synonyms: Phosphoinositide 3 Kinase Enhancer; Polyclonal Antibody; Phosphoinositide 3 Kinase Enhancer (PIKE; Centaurin gamma 1); Anti -Phosphoinositide 3 Kinase Enhancer (PIKE; anti-LOC100557420 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIKE. Will detect both PIKE-L and PIKE-A isoforms. Species Crossreactivity: Mouse.
• Purity/Purification: Highly Purified; Purified by Ion Exchange Chromatography.
• Form/Format: Supplied as a liquid in PBS with 0.02% sodium azide.

• Applicable Applications for anti-LOC100557420 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry: Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10ug/ml.
• Immunogen: Peptide corresponding to 15 amino acids near the C-terminus of human PIKE (GenBank accession no. AAM97540)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of PIKE in mouse brain cell lysates with MBS617095 at (A) 0.5, (B) 1, and (C) 2 ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10ug/ml.)

Related Product Information for anti-LOC100557420 antibody

Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity (1). PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1 (reviewed in 2). The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis (3). Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes (4). In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

Product Categories/Family for anti-LOC100557420 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 327268337
• NCBI GeneID: 100557420
• NCBI Accession #: XP_003218954.1
• NCBI GenBank Nucleotide #: XM_003218906.1
• NCBI Official Full Name: connector enhancer of kinase suppressor of ras 2-like isoform 3
• NCBI Official Symbol: LOC100557420
• NCBI Protein Information: connector enhancer of kinase suppressor of ras 2-like