SDS-Page
(WB analysis of purified beta-Phosphoglucomutase under reducing and non-reducing conditions: Reduced samples of protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of MBS610799(overnight at 4°C). Dylight 488-conjugated donkey anti goat IgG was used for visualization.)
Goat Phosphoglucomutase, beta Polyclonal Antibody | anti-PGM antibody
Phosphoglucomutase, beta (PGM)
Gene Names
PGM; ARABIDOPSIS THALIANA PHOSPHOGLUCOMUTASE; ATPGMP; MIO24.4; MIO24_4; PGM; PGM1; phosphoglucomutase; PHOSPHOGLUCOMUTASE; STARCH-FREE 1; STF1
Applications
ELISA, Western Blot, Gel Super Shift Assay
Purity
Highly PurifiedPurified from monospecific antiserum by salt fractionation and ion exchange chromatography.
Synonyms
Phosphoglucomutase; beta; Polyclonal Antibody; beta (PGM); Anti -Phosphoglucomutase; anti-PGM antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the 34kD enzyme. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified b-Phosphoglucomutase.
Purity/Purification
Highly Purified
Purified from monospecific antiserum by salt fractionation and ion exchange chromatography.
Purified from monospecific antiserum by salt fractionation and ion exchange chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Applicable Applications for anti-PGM antibody
ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for Immunoblotting (Western or Dot Blot), ELISA, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Dilution: ELISA: 1:10,000 to 1:50,000. Against 1ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using Peroxidase conjugated rabbit anti-goat IgG [H&L] and ABTS as a substrate for 30 minutes at RT.
Dilution: ELISA: 1:10,000 to 1:50,000. Against 1ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using Peroxidase conjugated rabbit anti-goat IgG [H&L] and ABTS as a substrate for 30 minutes at RT.
Immunogen
Beta Phosphoglucomutase [Lactococcus lacti]
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(WB analysis of purified beta-Phosphoglucomutase under reducing and non-reducing conditions: Reduced samples of protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of MBS610799(overnight at 4°C). Dylight 488-conjugated donkey anti goat IgG was used for visualization.)
SDS-Page
(WB analysis of purified beta-Phosphoglucomutase under reducing and non-reducing conditions: Reduced samples of protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of MBS610799(overnight at 4°C). Dylight 488-conjugated donkey anti goat IgG was used for visualization.)
Related Product Information for anti-PGM antibody
Phosphoglucomutase (PGM) interconverts the 1- and 6-phosphate isomers of alpha-D-glucose. Although it is a reversible reaction, the formation of glucose-6-phosphate is markedly favored. PGM will not convert beta-D-glucose-1-phosphate or beta-D-glucose-6-phosphate.
Product Categories/Family for anti-PGM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,989 Da
NCBI Official Full Name
phosphoglucomutase
NCBI Official Symbol
PGM
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA PHOSPHOGLUCOMUTASE; ATPGMP; MIO24.4; MIO24_4; PGM; PGM1; phosphoglucomutase; PHOSPHOGLUCOMUTASE; STARCH-FREE 1; STF1
NCBI Protein Information
phosphoglucomutase
UniProt Protein Name
Phosphoglucomutase, chloroplastic
Protein Family
UniProt Gene Name
PGMP
UniProt Synonym Gene Names
PGM; PGM
UniProt Entry Name
PGMP_ARATH
Uniprot Description
Function: This enzyme participates in both the breakdown and synthesis of glucose
By similarity.
Catalytic activity: Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.
Cofactor: Binds 1 magnesium ion per subunit
By similarity.
Subcellular location: Plastid › chloroplast.
Sequence similarities: Belongs to the phosphohexose mutase family.
Research Articles on PGM
Similar Products
Product Notes
The PGM pgmp (Catalog #AAA610799) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phosphoglucomutase, beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for Immunoblotting (Western or Dot Blot), ELISA, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Dilution: ELISA: 1:10,000 to 1:50,000. Against 1ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using Peroxidase conjugated rabbit anti-goat IgG [H&L] and ABTS as a substrate for 30 minutes at RT. Researchers should empirically determine the suitability of the PGM pgmp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphoglucomutase, beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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