Rabbit anti-Human, Rat Phosphodiesterase 4B Polyclonal Antibody | anti-PDE4B antibody

Phosphodiesterase 4B (PDE4B)

Cat. Num. AAA617794

• Gene Names: PDE4B; DPDE4; PDE4B5; PDEIVB; MGC126529; DKFZp686F2182
• Reactivity: Human, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Phosphodiesterase 4B; Polyclonal Antibody; Phosphodiesterase 4B (PDE4B); Anti -Phosphodiesterase 4B (PDE4B); anti-PDE4B antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: Reacts with the human and rat PDE4B protein. On Western blots, it identifies a single band at ~83kD. Bands of unknown origin may be observed at ~160 and ~260kD. Reactivities have been confirmed with NG96 human neuroblastoma lysate and rat brain homogenate.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-PDE4B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation and Western Blot.
• Immunogen: Synthetic peptide derived from the N-terminal region of human and rat PDE4B protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PDE4B antibody

Of the 11 families of phosphodiesterase (PDEs) identified, PDE4, PDE7, and PDE8 are specific for cAMP. However, isoforms of the PDE4 family often account for most of the cAMP-hydrolyzing activity of a cell. Four genes (PDE4A, B, C and D) make up the PDE4 family. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In the CNS, PDE4B subtype variants are expressed in varying ratios. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants.

Product Categories/Family for anti-PDE4B antibody

Antibodies; Abs to Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 119626930
• NCBI GeneID: 5142
• UniProt Accession #: Q07343; O15443; Q13945; Q59GM8; Q5TEK7; Q68CX5
• Molecular Weight: 83,343 Da
• NCBI Official Full Name: phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila), isoform CRA_b
• NCBI Official Synonym Full Names: phosphodiesterase 4B, cAMP-specific
• NCBI Official Symbol: PDE4B
• NCBI Official Synonym Symbols: DPDE4; PDE4B5; PDEIVB; MGC126529; DKFZp686F2182
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4B; PDE32; OTTHUMP00000010652; OTTHUMP00000010653; OTTHUMP00000010654; OTTHUMP00000010656; OTTHUMP00000232365; dunce-like phosphodiesterase E4; cAMP-specific phosphodiesterase-4 B isoform; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE4B
• UniProt Synonym Gene Names: DPDE4
• UniProt Entry Name: PDE4B_HUMAN

NCBI Description

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Uniprot Description

PDE4B: a cAMP-specific phosphodiesterase. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. Inhibited by rolipram. Three alternatively-spliced isoforms have been described.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: perinuclear region of cytoplasm; voltage-gated calcium channel complex; cytosol; Z disc; nucleus

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; metal ion binding; cAMP binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: neutrophil chemotaxis; positive regulation of interferon-gamma production; cAMP catabolic process; positive regulation of interleukin-2 production; neutrophil homeostasis; leukocyte migration; T cell receptor signaling pathway