Rabbit anti-Human MDC1 Polyclonal Antibody | anti-MDC1 antibody

MDC1 (Phospho-Ser513) Antibody

Cat. Num. AAA9414687

• Gene Names: MDC1; NFBD1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: MDC1; Polyclonal Antibody; MDC1 (Phospho-Ser513) Antibody; IER3; NFBD1; KIAA0170; DKFZp781A0122; anti-MDC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of MDC1 only when phosphorylated at serine 513.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2089

• Applicable Applications for anti-MDC1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 513(E-R-S(p)-Q-A) derived from Human MDC1.
• Modification: Phospho-Ser513
• Target Name: MDC1
• Preparation and Storage: Store at -20 degree C/1 year

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using MDC1 (Phospho-Ser513) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-MDC1 antibody

The MDC1 protein contains a forkhead homology-associated (FHA) domain and 2 BRCA1 (MIM 113705) C-terminal motifs (BRCTs) and is required for the intra-S-phase DNA damage checkpoint.Nagase T., DNA Res. 3:17-24(1996).Shiina T., Genetics 173:1555-1570(2006).Mungall A.J., Nature 425:805-811(2003).

Product Categories/Family for anti-MDC1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 132626688
• NCBI GeneID: 9656
• NCBI Accession #: NP_055456.2
• NCBI GenBank Nucleotide #: NM_014641.2
• UniProt Accession #: Q14676; Q0EFC2; Q2L6H7; Q2TAZ4; Q5JP55; Q5JP56; Q5ST83; A2AB04; A2BF04; A2RRA8; A7YY86; B0S8A2
• Molecular Weight: 116,634 Da
• NCBI Official Full Name: mediator of DNA damage checkpoint protein 1
• NCBI Official Synonym Full Names: mediator of DNA-damage checkpoint 1
• NCBI Official Symbol: MDC1
• NCBI Official Synonym Symbols: NFBD1
• NCBI Protein Information: mediator of DNA damage checkpoint protein 1
• UniProt Protein Name: Mediator of DNA damage checkpoint protein 1
• Protein Family: Mediator of DNA damage checkpoint protein
• UniProt Gene Name: MDC1
• UniProt Synonym Gene Names: KIAA0170; NFBD1
• UniProt Entry Name: MDC1_HUMAN

NCBI Description

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]

Uniprot Description

MDC1: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; focal adhesion; chromosome; nucleus

Molecular Function: protein C-terminus binding; protein binding

Biological Process: intra-S DNA damage checkpoint; double-strand break repair; DNA repair; double-strand break repair via homologous recombination