Western Blot (WB)
(Western Blot; Sample: Recombinant PDE3A, Rat.)
Rabbit anti-Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) Polyclonal Antibody | anti-PDE3A antibody
Polyclonal Antibody to Phosphodiesterase 3A, cGMP Inhibited (PDE3A)
Gene Names
Pde3a; RNPDE3A
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Phosphodiesterase 3A; cGMP Inhibited (PDE3A); Polyclonal Antibody; Polyclonal Antibody to Phosphodiesterase 3A; CGI-PDE; Cyclic GMP-inhibited phosphodiesterase A; cGMP-inhibited 3'; 5'-cyclic phosphodiesterase A; anti-PDE3A antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PDE3A. It has been selected for its ability to recognize PDE3A in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Concentration
0.71mg/ml (varies by lot)
Sequence
Leu948~Leu1141
Applicable Applications for anti-PDE3A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:350-1400
IHC: 5-20ug/mL; 1:35-140
ICC: 5-20ug/mL; 1:35-140
IHC: 5-20ug/mL; 1:35-140
ICC: 5-20ug/mL; 1:35-140
Immunogen Sequence
Leu948~Leu1141
Cross Reactivity
Rat
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant PDE3A, Rat.)
Western Blot (WB)
(Western Blot; Sample: Recombinant PDE3A, Rat.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue)
Product Categories/Family for anti-PDE3A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
124,301 Da
NCBI Official Full Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase A
NCBI Official Synonym Full Names
phosphodiesterase 3A
NCBI Official Symbol
Pde3a
NCBI Official Synonym Symbols
RNPDE3A
NCBI Protein Information
cGMP-inhibited 3',5'-cyclic phosphodiesterase A
UniProt Protein Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Protein Family
UniProt Gene Name
Pde3a
UniProt Synonym Gene Names
CGI-PDE A
NCBI Description
plays a role in cyclic nucleotide degradation; may be involved in oocyte maturation [RGD, Feb 2006]
Uniprot Description
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
Research Articles on PDE3A
Similar Products
Product Notes
The PDE3A pde3a (Catalog #AAA2112460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphodiesterase 3A, cGMP Inhibited (PDE3A) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphodiesterase 3A, cGMP Inhibited (PDE3A) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:350-1400 IHC: 5-20ug/mL; 1:35-140 ICC: 5-20ug/mL; 1:35-140. Researchers should empirically determine the suitability of the PDE3A pde3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Leu948~Leu 1141. It is sometimes possible for the material contained within the vial of "Phosphodiesterase 3A, cGMP Inhibited (PDE3A), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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