Phosphodiesterase 3A Recombinant Protein | PDE3A recombinant protein

Recombinant Phosphodiesterase 3A, cGMP Inhibited (PDE3A)

Cat. Num. AAA2034653

• Gene Names: PDE3A; HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A
• Reactivity: Rat
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Phosphodiesterase 3A; Recombinant Phosphodiesterase 3A; cGMP Inhibited (PDE3A); Cyclic GMP-inhibited phosphodiesterase A; cGMP-inhibited 3'; 5'-cyclic phosphodiesterase A; PDE3A recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Rat
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 1141

• Applicable Applications for PDE3A recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Leu948~Leu1141
• Tag: Two N-terminal Tags, His-tag and GST-tag
• Residues & Tags: Leu948~Leu1141 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 3059109
• NCBI GeneID: 5139
• UniProt Accession #: Q14432; O60865; Q13348; Q17RD1
• Molecular Weight: Predicted: 51.9kDa
• NCBI Official Full Name: phosphodiesterase 3A
• NCBI Official Synonym Full Names: phosphodiesterase 3A
• NCBI Official Symbol: PDE3A
• NCBI Official Synonym Symbols: HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• Protein Family: cGMP-inhibited 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE3A
• UniProt Synonym Gene Names: CGI-PDE A
• UniProt Entry Name: PDE3A_HUMAN

NCBI Description

This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

PDE3A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Inhibited by cGMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

Protein type: Membrane protein, multi-pass; EC 3.1.4.17; Phosphodiesterase; Membrane protein, integral; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity

Biological Process: cAMP catabolic process; cAMP-mediated signaling; cGMP-mediated signaling; lipid metabolic process; negative regulation of vascular permeability; positive regulation of vascular permeability

Disease: Hypertension And Brachydactyly Syndrome