Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-YAP1-S128 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)
Rabbit anti-Human, Mouse Phospho-YAP1-S128 Polyclonal Antibody | anti-YAP1-S128 antibody
Phospho-YAP1-S128 Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-YAP1-S128 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-YAP1-S128 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq, Jul 2008]
Uniprot Description
YAP1: an adaptor protein that binds to HER4 and the SH3 domain of the Yes tyrosine kinase. Associates with multiple transcription factors in the nucleus, and appears to be a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. Contains a WW domain that is found in various structural, regulatory and signaling molecules.
Protein type: Oncoprotein; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; transcription factor complex; membrane; cytoplasm; cell junction; cytosol; nucleus
Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription corepressor activity
Biological Process: paraxial mesoderm development; transcription initiation from RNA polymerase II promoter; somatic stem cell maintenance; lateral mesoderm development; cell morphogenesis; negative regulation of epithelial cell differentiation; notochord development; positive regulation of organ growth; keratinocyte differentiation; cell proliferation; positive regulation of cell proliferation; gene expression; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis; response to DNA damage stimulus
Disease: Coloboma, Ocular, With Or Without Hearing Impairment, Cleft Lip/palate, And/or Mental Retardation
Research Articles on YAP1-S128
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Product Notes
The YAP1-S128 yap1 (Catalog #AAA9143894) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-YAP1-S128 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-YAP1-S128 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the YAP1-S128 yap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-YAP1-S128, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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