Rabbit anti-Human Phospho-MYT1 (T495) Polyclonal Antibody | anti-PKMYT1 antibody

Phospho-MYT1 (T495) Antibody

Cat. Num. AAA9204935

• Gene Names: PKMYT1; MYT1; PPP1R126
• Reactivity: Human
• Applications: Dot Blot, ELISA, Western Blot, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-MYT1 (T495); Polyclonal Antibody; Phospho-MYT1 (T495) Antibody; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1; anti-PKMYT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MYT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T495 of human MYT1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 430

• Applicable Applications for anti-PKMYT1 antibody: Dot Blot (DB), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: DB~~1:500; WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-MYT1-T495 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Western Blot (WB)

(The anti-Phospho-MYT1-T495 Antibody is used in Western blot to detect Phospho-MYT1-T495 in HL60 (left) and SK-BR-3 (right) tissue lysates.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PKMYT1 antibody

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-PKMYT1 antibody

Cell Biology; Signal Transduction; Phospho Antibodies

References

Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004). Nakajima, H., et al., J. Biol. Chem. 278(28):25277-25280 (2003). Passer, B.J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2284-2289 (2003). Okumura, E., et al., Nat. Cell Biol. 4(2):111-116 (2002). Booher, R.N., et al., J. Biol. Chem. 272(35):22300-22306 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 386642875
• NCBI GeneID: 9088
• NCBI Accession #: NP_001245379.1; NP_001245380.1; NP_004194.3; NP_872629.1
• NCBI GenBank Nucleotide #: NM_001258450.1
• UniProt Accession #: Q99640; O14731; Q7LE24; Q8TCM9; B3KUN8; B4DXD4; D3DUA4; F8W164; I3L1V2
• Molecular Weight: 54521
• NCBI Official Full Name: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 3
• NCBI Official Synonym Full Names: protein kinase, membrane associated tyrosine/threonine 1
• NCBI Official Symbol: PKMYT1
• NCBI Official Synonym Symbols: MYT1; PPP1R126
• NCBI Protein Information: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Protein Family: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Gene Name: PKMYT1
• UniProt Synonym Gene Names: MYT1
• UniProt Entry Name: PMYT1_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Myt1: a dual specificity protein kinase of the WEE family. Preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Alternative splice-variant isoforms have been reported.

Protein type: Protein kinase, dual-specificity (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Other group; WEE family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; kinase activity; ATP binding; protein kinase activity

Biological Process: mitosis; regulation of cell cycle; regulation of mitosis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle