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Western Blot (WB) (Staining (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)

Goat PHEMX/TSPAN32 Polyclonal Antibody | anti-TSPAN32 antibody

Goat anti-PHEMX/TSPAN32 Antibody

Gene Names
TSPAN32; ART1; PHMX; PHEMX; TSSC6
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PHEMX/TSPAN32; Polyclonal Antibody; Goat anti-PHEMX/TSPAN32 Antibody; TSPAN32; PHEMX; tetraspanin 32; MGC22455; PHMX; TSSC6; pan-hematopoietic expression; pan-hematopoietic expression protein; tetraspanin; tumor-suppressing STF cDNA 6; tumor-suppressing subchromosomal transferable fragment cDNA 6; tumor-suppressing subtrans; TSPAN32 antibody; PHEMX antibody; tetraspanin 32 antibody; MGC22455 antibody; PHMX antibody; TSSC6 antibody; pan-hematopoietic expression antibody; pan-hematopoietic expression protein antibody; tetraspanin antibody; tumor-suppressing STF cDNA 6 antibody; tumor-suppressing subchromosomal transferable fragment cDNA 6 antibody; tumor-suppressing subtransferable candidate 6 antibody; ART1 antibody; FLJ17158 antibody; FLJ97586 antibody; PHEMX / TSPAN32; anti-TSPAN32 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
GGLSGCPERGLSD
Sequence Length
320
Applicable Applications for anti-TSPAN32 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 33-35kDa band seen in HeLa lysate. Recommended for use at 1-3ug/ml.
Immunogen
Peptide with sequence GGLSGCPERGLSD, from the C Terminus of the protein sequence according to NP_620591.3.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(Staining (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB) (Staining (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,211 Da
NCBI Official Full Name
tetraspanin-32
NCBI Official Synonym Full Names
tetraspanin 32
NCBI Official Symbol
TSPAN32
NCBI Official Synonym Symbols
ART1; PHMX; PHEMX; TSSC6
NCBI Protein Information
tetraspanin-32
UniProt Protein Name
Tetraspanin-32
Protein Family
UniProt Gene Name
TSPAN32
UniProt Synonym Gene Names
PHEMX; TSSC6; Tspan-32
UniProt Entry Name
TSN32_HUMAN

NCBI Description

This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TSPAN32: This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cell surface; integral to plasma membrane; intracellular

Biological Process: cell surface receptor linked signal transduction; cell-cell signaling; cytoskeleton organization and biogenesis; defense response to protozoan; integrin-mediated signaling pathway; negative regulation of cell proliferation; negative regulation of myeloid dendritic cell activation; regulation of defense response to virus

Research Articles on TSPAN32

Similar Products

Product Notes

The TSPAN32 tspan32 (Catalog #AAA420044) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PHEMX/TSPAN32 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHEMX/TSPAN32 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 33-35kDa band seen in HeLa lysate. Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the TSPAN32 tspan32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GGLSGCPERG LSD. It is sometimes possible for the material contained within the vial of "PHEMX/TSPAN32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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