anti-Human PHD2 Polyclonal Antibody | anti-PHD2 antibody
Anti - PHD2 (PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, EGLN1, egl nine homolog 1, C1orf12, chromosome 1 open reading frame 12)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
PHD2; Polyclonal Antibody; Anti - PHD2 (PH2; Prolyl hydroxylase domain containing protein 2; HIF2PH2; HIF-Prolyl hydroxylase 2; EGLN1; egl nine homolog 1; C1orf12; chromosome 1 open reading frame 12); PHD2 (PH2; anti-PHD2 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PHD2.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Concentration
~1mg/ml (varies by lot)
Sequence Length
252
Applicable Applications for anti-PHD2 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA and Western Blot. Other applications not tested.
Dilution: ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well. See P3374-05A.
Western Blot: 1-5ug/ml using ECL technique.
Optimal dilutions to be determined by the researcher.
Dilution: ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well. See P3374-05A.
Western Blot: 1-5ug/ml using ECL technique.
Optimal dilutions to be determined by the researcher.
Immunogen
16aa peptide of human PHD2/EGLN1. Location
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-PHD2 antibody
Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.
Product Categories/Family for anti-PHD2 antibody
References
• Dupuy D et al (2000) Genomincs 69, 348-354; Taylor MS et al (2001) Gene 275, 125-132; Semenza GL et al (2001) Cell, 107, 1-3; Epstein ACR et al (2001) Cell 107, 43-54; Ivan M et al (2002) PNAS 99, 13459-13464; Cioffi CL et al BBRC 303, 947-953
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PHD2 (Catalog #AAA612729) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - PHD2 (PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, EGLN1, egl nine homolog 1, C1orf12, chromosome 1 open reading frame 12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHD2 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA and Western Blot. Other applications not tested. Dilution: ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well. See P3374-05A. Western Blot: 1-5ug/ml using ECL technique. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the PHD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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