Immunofluorescence (IF)
(Immunofluorescence analysis: HeLa cells were stained with MBS609533 (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).)
Mouse anti-Mouse PHD2 Monoclonal Antibody
PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20)
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunofluorescence
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
PHD2; Monoclonal Antibody; PHD2 (PH2; Prolyl Hydroxylase Domain Containing Protein 2; HIF2PH2; HIF-Prolyl Hydroxylase 2; EGLN1; Egl Nine Homolog 1; C1orf12; Chromosome 1 Open Reading Frame 12; PNAS-118; PNAS-137; Hypoxia-inducible Factor Prolyl Hydroxylase 2; SM-20); Anti -PHD2 (PH2; anti-PHD2 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
10K404
Specificity
Recognizes human PHD2.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-PHD2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA, and Western Blot.
Dilution: Immunofluorescence: 1:500-1:1,000
Western Blot:1:500-1:1,000
Dilution: Immunofluorescence: 1:500-1:1,000
Western Blot:1:500-1:1,000
Immunogen
Recombinant human PHD2 (aa271-426) purified from E.coli (Accession #NP_071334).
Hybridoma
Mouse F0 myeloma cells with spleen cells from BALB/c mice.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunofluorescence (IF)
(Immunofluorescence analysis: HeLa cells were stained with MBS609533 (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).)
Immunofluorescence (IF)
(Immunofluorescence analysis: HeLa cells were stained with MBS609533 (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).)
Western Blot (WB)
(Western blot analysis: Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with MBS609533 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Western blot analysis: Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with MBS609533 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-PHD2 antibody
PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. EGLN family, which is known to be cellular oxygen sensor, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.
Product Categories/Family for anti-PHD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
PHD2
Similar Products
Product Notes
The PHD2 (Catalog #AAA609533) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PHD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, and Western Blot. Dilution: Immunofluorescence: 1:500-1:1,000 Western Blot:1:500-1:1,000. Researchers should empirically determine the suitability of the PHD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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