Rabbit anti-Human EGLN2 Polyclonal Antibody | anti-EGLN2 antibody

EGLN2, ID (EGLN2, EIT6, Egl nine homolog 2, Estrogen-induced tag 6, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 1, Prolyl hydroxylase domain-containing protein 1) (PE)

Cat. Num. AAA6294730

• Gene Names: EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EGLN2; Polyclonal Antibody; ID (EGLN2; EIT6; Egl nine homolog 2; Estrogen-induced tag 6; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; Prolyl hydroxylase domain-containing protein 1) (PE); Prolyl hydroxylase domain-containing protein 1; anti-EGLN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EGLN2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EGLN2 antibody is generated from rabbits immunized with a KLH conjugated Synthetic peptide selected from the center region of human EGLN2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EGLN2 Antibody (Center) in MDA-MB231, Hela cell line lysates (35ug/lane). EGLN2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with EGLN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EGLN2 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EGLN2 antibody

EGLN2 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.

Product Categories/Family for anti-EGLN2 antibody

Antibodies; Hypoxia

References

Semenza, G.L. et.al., Cell 107 (1), 1-3 (2001). Aprelikova, O., et.al., Cancer Res. 69 (2), 616-624 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 32129513
• NCBI GeneID: 112398
• UniProt Accession #: Q96KS0; Q8WWY4; Q9BV14; A8K5S0
• Molecular Weight: 407
• NCBI Official Full Name: Egl nine homolog 2
• NCBI Official Synonym Full Names: egl-9 family hypoxia-inducible factor 2
• NCBI Official Symbol: EGLN2
• NCBI Official Synonym Symbols: EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
• NCBI Protein Information: egl nine homolog 2; estrogen-induced tag 6; HIF-prolyl hydroxylase 1; hypoxia-inducible factor prolyl hydroxylase 1; prolyl hydroxylase domain-containing protein 1
• UniProt Protein Name: Egl nine homolog 2
• Protein Family: Egl nine
• UniProt Gene Name: EGLN2
• UniProt Synonym Gene Names: EIT6; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; PHD1
• UniProt Entry Name: EGLN2_HUMAN

NCBI Description

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

Uniprot Description

EGLN2: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 1.14.11.29; Oxidoreductase

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function: protein binding; oxygen sensor activity; L-ascorbic acid binding; ferrous iron binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity

Biological Process: estrogen receptor signaling pathway; cell redox homeostasis; regulation of neuron apoptosis; positive regulation of protein catabolic process; response to hypoxia; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; regulation of cell growth