Rabbit anti-Human PHD1 Polyclonal Antibody | anti-PHD1 antibody

PHD1 (PH1, prolyl hydroxylase domain-containing protein 1, HIFPH1, HIF-prolyl hydroxylase 1, EGLN2, EGL nine homolog 2, EIT6, estrogen-induced tag 6, DKFZp434E026)

Cat. Num. AAA611615

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PHD1; Polyclonal Antibody; PHD1 (PH1; prolyl hydroxylase domain-containing protein 1; HIFPH1; HIF-prolyl hydroxylase 1; EGLN2; EGL nine homolog 2; EIT6; estrogen-induced tag 6; DKFZp434E026); Anti -PHD1 (PH1; anti-PHD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHD1. Species Sequence Homology: mouse, rat - 95%. No significant sequence homology exist with other PHDs.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 40% glycerol.

• Applicable Applications for anti-PHD1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:10,000-1:100,000; Using 50-100ng of P3373-05A control peptide/well.; Western Blot: 1-5ug/ml using ECL technique.
• Immunogen: A synthetic 22aa peptide of the C-terminus human PHD1/EGLN2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PHD1 antibody

Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.

Product Categories/Family for anti-PHD1 antibody

Antibodies; Abs to Hypoxia

NCBI and Uniprot Product Information

• NCBI GI #: 486056
• UniProt Accession #: P36093; Q2VQ88; D6VXP3
• Molecular Weight: 40,646 Da
• NCBI Official Full Name: PHD1
• UniProt Protein Name: Putative transcription factor PHD1
• Protein Family: Putative transcription factor
• UniProt Gene Name: PHD1
• UniProt Entry Name: PHD1_YEAST

Uniprot Description

Function: Putative transcription factor that functions in pseudohyphal growth.

Subcellular location: Nucleus.

Miscellaneous: Present with 1420 molecules/cell in log phase SD medium. Ref.7

Sequence similarities: Belongs to the EFG1/PHD1/stuA family.Contains 1 HTH APSES-type DNA-binding domain.