Western Blot (WB)
(ANP32B Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ANP32B antibody detected the ANP32B protein (arrow).)
Rabbit anti-Human PHAPI2 Polyclonal Antibody | anti-PHAPI2 antibody
PHAPI2 (Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member B, Acidic Protein Rich in Leucines, Putative HLA-DR-associated Protein I-2, ANP32B, Silver-stainable Protein SSP29, APRIL) (PE)
Gene Names
ANP32B; APRIL; SSP29; PHAPI2
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PHAPI2; Polyclonal Antibody; PHAPI2 (Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member B; Acidic Protein Rich in Leucines; Putative HLA-DR-associated Protein I-2; ANP32B; Silver-stainable Protein SSP29; APRIL) (PE); APRIL; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Acidic protein rich in leucines; Putative HLA-DR-associated protein I-2; Silver-stainable protein SSP29; anti-PHAPI2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PHAPI2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
ANP32B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human ANP32B.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ANP32B Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ANP32B antibody detected the ANP32B protein (arrow).)
Western Blot (WB)
(ANP32B Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ANP32B antibody detected the ANP32B protein (arrow).)
Related Product Information for anti-PHAPI2 antibody
Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity).
Product Categories/Family for anti-PHAPI2 antibody
References
Tochio, N., et al. J. Mol. Biol. 401(1):97-114(2010). Shen, S.M., et al. Carcinogenesis 31(3):419-426(2010). Chemnitz, J., et al. Eur. J. Immunol. 39(1):267-279(2009). Munemasa, Y., et al. Mol. Cell. Biol. 28(3):1171-1181(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,788 Da
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member B
NCBI Official Synonym Full Names
acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
NCBI Official Symbol
ANP32B
NCBI Official Synonym Symbols
APRIL; SSP29; PHAPI2
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member B; silver-stainable protein SSP29; acidic protein rich in leucines; putative HLA-DR-associated protein I-2
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member B
UniProt Gene Name
ANP32B
UniProt Synonym Gene Names
APRIL; PHAPI2; PHAPI2
UniProt Entry Name
AN32B_HUMAN
Uniprot Description
ANP32B: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity. Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Belongs to the ANP32 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Chaperone
Chromosomal Location of Human Ortholog: 9q22.32
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: histone binding
Biological Process: positive regulation of protein export from nucleus; caspase activation; nucleosome assembly; negative regulation of cell differentiation; vasculature development; ventricular system development; palate development; inner ear development
Research Articles on PHAPI2
Similar Products
Product Notes
The PHAPI2 anp32b (Catalog #AAA6273850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHAPI2 (Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member B, Acidic Protein Rich in Leucines, Putative HLA-DR-associated Protein I-2, ANP32B, Silver-stainable Protein SSP29, APRIL) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHAPI2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PHAPI2 anp32b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHAPI2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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