Rabbit anti-Human, Mouse RGS19 Polyclonal Antibody | anti-RGS19 antibody

RGS19 (S151) (RGS19, GAIP, GNAI3IP, Regulator of G-protein signaling 19, G-alpha-interacting protein) (APC)

Cat. Num. AAA6341720

• Gene Names: RGS19; GAIP; RGSGAIP
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RGS19; Polyclonal Antibody; RGS19 (S151) (RGS19; GAIP; GNAI3IP; Regulator of G-protein signaling 19; G-alpha-interacting protein) (APC); G-alpha-interacting protein; anti-RGS19 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RGS19 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: RGS19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136~166 amino acids surrounding S151 of human RGS19.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-RGS19 Antibody (S151) in mouse muscle tissue lysates (35ug/lane). RGS19(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Phospho-RGS19-pS151.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-RGS19 antibody

RGS19 enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, which stimulates autophagy by favoring the GDP-bound form of Galphai3. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Product Categories/Family for anti-RGS19 antibody

Antibodies; G Proteins

References

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). Greenberg JT. Dev Cell. 8(6):799-801. (2005). Levine B. Cell. 120(2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004). de Vries L., et al. PNAS 93:15203-15208(1996). de Alba E., et al. J. Mol. Biol. 291:927-939(1999). Wang J., et al. J. Biol. Chem. 273:26014-26025(1998). Ogier-Denis E., et al. J. Biol. Chem. 275:39090-39095(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 86990435
• NCBI GeneID: 10287
• NCBI Accession #: NP_001034556.1
• NCBI GenBank Nucleotide #: NM_001039467.1
• UniProt Accession #: P49795; Q53XN0; Q8TD60; A8K216; E1P5G9
• Molecular Weight: 24,636 Da
• NCBI Official Full Name: regulator of G-protein signaling 19
• NCBI Official Synonym Full Names: regulator of G-protein signaling 19
• NCBI Official Symbol: RGS19
• NCBI Official Synonym Symbols: GAIP; RGSGAIP
• NCBI Protein Information: regulator of G-protein signaling 19; G alpha interacting protein; G protein signalling regulator 19; G-alpha-interacting protein; guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein; regulator of G-protein signal
• UniProt Protein Name: Regulator of G-protein signaling 19
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS19
• UniProt Synonym Gene Names: GAIP; GNAI3IP; RGS19; GAIP
• UniProt Entry Name: RGS19_HUMAN

NCBI Description

G proteins mediate a number of cellular processes. The protein encoded by this gene belongs to the RGS (regulators of G-protein signaling) family and specifically interacts with G protein, GAI3. This protein is a guanosine triphosphatase-activating protein that functions to down-regulate Galpha i/Galpha q-linked signaling. Alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS19: inhibits signal transduction by increasing the GTPase activity of g protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(I)a3 > G(I)a1 > G(O)a >> G(Z)a/G(I)a2. Activity on G(Z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: Golgi apparatus; clathrin-coated vesicle; membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; brush border

Molecular Function: protein binding; G-protein alpha-subunit binding; GTPase activator activity

Biological Process: G-protein coupled receptor protein signaling pathway; small GTPase mediated signal transduction; autophagy; positive regulation of GTPase activity