Western Blot (WB)
(Western blot analysis of PGHS-2 expression in HeLa (A), Jurkat (B), Raw264.7 (C), rat lung (D) whole cell lysates.)
Rabbit PGHS-2 Polyclonal Antibody | anti-PTGS2 antibody
Anti-PGHS-2 Antibody
Western Blot (WB)
(Western blot analysis of PGHS-2 expression in HeLa (A), Jurkat (B), Raw264.7 (C), rat lung (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PGHS-2 expression in HeLa (A), Jurkat (B), Raw264.7 (C), rat lung (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PGHS-2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PGHS-2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of PGHS-2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of PGHS-2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Uniprot Description
COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.
Protein type: Oxidoreductase; EC 1.14.99.1; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 1q25.2-q25.3
Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus
Molecular Function: prostaglandin-endoperoxide synthase activity; enzyme binding; protein homodimerization activity; peroxidase activity; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding
Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; response to lithium ion; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; decidualization; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission, dopaminergic; bone mineralization; response to estradiol stimulus; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; regulation of blood pressure; negative regulation of smooth muscle contraction; arachidonic acid metabolic process; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; response to fructose stimulus; water-soluble vitamin metabolic process; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; hair cycle; prostaglandin metabolic process; ovulation; response to manganese ion; NAD metabolic process; regulation of inflammatory response; positive regulation of vasoconstriction; brown fat cell differentiation; positive regulation of fever; response to oxidative stress; cell motility; embryo implantation
Research Articles on PTGS2
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Product Notes
The PTGS2 ptgs2 (Catalog #AAA820831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGHS-2 Antibody reacts with Human, Mouse, Rat, Bovine, Pig, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's PGHS-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the PTGS2 ptgs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGHS-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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