Goat PGBD2 Polyclonal Antibody | anti-PGBD2 antibody

Goat anti-PGBD2 Antibody

Cat. Num. AAA420236

• Reactivity: Expected from sequence similarity: Human
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PGBD2; Polyclonal Antibody; Goat anti-PGBD2 Antibody; piggyBac transposable element derived 2; hypothetical protein LOC267002; PGBD2 antibody; piggyBac transposable element derived 2 antibody; hypothetical protein LOC267002 antibody; anti-PGBD2 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ESNADTTSQGRRSRR
• Sequence Length: 341

• Applicable Applications for anti-PGBD2 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Preliminary experiments gave an approx 30kDa band in Human Brain (Cerebral Cortex) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 39.6kDa according to NP_001017434.1 and of 68. 0kDa according to NP_733843.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-ESNADTTSQGRRSRR, from the internal region of the protein sequence according to NP_001017434.1; NP_733843.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise both reported isoforms (NP_001017434.1; NP_733843.1)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 62953123
• NCBI GeneID: 267002
• NCBI Accession #: NP_001017434.1; NP_733843.1
• NCBI GenBank Nucleotide #: NM_001017434.1
• Molecular Weight: 39,563 Da
• NCBI Official Full Name: piggyBac transposable element-derived protein 2 isoform b
• NCBI Official Synonym Full Names: piggyBac transposable element derived 2
• NCBI Official Symbol: PGBD2
• NCBI Protein Information: piggyBac transposable element-derived protein 2
• UniProt Protein Name: PiggyBac transposable element-derived protein 2
• Protein Family: PiggyBac transposable element-derived protein
• UniProt Gene Name: PGBD2
• UniProt Entry Name: PGBD2_HUMAN

NCBI Description

The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. The exact function of this gene is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PGBD2: 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q44