Western Blot (WB)
(The PFKM polyclonal antibody is used in Western blot to detect PFKM in Ramos cell lysate.)
Rabbit anti-Human PFKM Polyclonal Antibody | anti-PFKM antibody
PFKM, NT (6-phosphofructokinase, Muscle Type, Phosphofructokinase 1, Phosphohexokinase, Phosphofructo-1-kinase Isozyme A, PFK-A, Phosphofructokinase-M, PFKX)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Western Blot (WB)
(The PFKM polyclonal antibody is used in Western blot to detect PFKM in Ramos cell lysate.)
Western Blot (WB)
(The PFKM polyclonal antibody is used in Western blot to detect PFKM in Ramos cell lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
NCBI and Uniprot Product Information
NCBI Description
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
Uniprot Description
PFKM: phosphofructokinase, muscle type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Defects in PFKM are the cause of glycogen storage disease VII (GSD-VII) also known as Tarui disease. Two alternatively spliced isoforms have been described.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; EC 2.7.1.11; Carbohydrate Metabolism - pentose phosphate pathway; Kinase, other; Carbohydrate Metabolism - fructose and mannose
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: 6-phosphofructokinase complex; apical plasma membrane; cytosol
Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein homodimerization activity; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity
Biological Process: muscle maintenance; glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; positive regulation of insulin secretion; glucose metabolic process; pathogenesis; glucose homeostasis; fructose 6-phosphate metabolic process; protein oligomerization
Disease: Glycogen Storage Disease Vii
Research Articles on PFKM
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Product Notes
The PFKM pfkm (Catalog #AAA628336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKM, NT (6-phosphofructokinase, Muscle Type, Phosphofructokinase 1, Phosphohexokinase, Phosphofructo-1-kinase Isozyme A, PFK-A, Phosphofructokinase-M, PFKX) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFKM can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the PFKM pfkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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