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Western Blot (WB) (Anti-LPGAT1 Antibody (Center) at 1:2000 dilution + Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human LPGAT1 Polyclonal Antibody | anti-LPGAT1 antibody

LPGAT1 Antibody (Center)

Gene Names
LPGAT1; NET8; FAM34A; FAM34A1
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
LPGAT1; Polyclonal Antibody; LPGAT1 Antibody (Center); Acyl-CoA:lysophosphatidylglycerol acyltransferase 1; 2.3.1.-; FAM34A; KIAA0205; anti-LPGAT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This LPGAT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 144-177 amino acids from the Central region of human LPGAT1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
370
Applicable Applications for anti-LPGAT1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Location
Highly expressed in liver and placenta. Also expressed in peripheral blood, lung, kidney and brain. Detected at lower levels in colon.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-LPGAT1 Antibody (Center) at 1:2000 dilution + Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-LPGAT1 Antibody (Center) at 1:2000 dilution + Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-LPGAT1 antibody
Lysophoshatidylglycerol (LPG) specific acyltransferase that recognizes various acyl-CoAs and LPGs as substrates but demonstrates a clear preference for long chain saturated fatty acyl-CoAs and oleoyl-CoA as acyl donors. Prefers oleoyl-LPG over palmitoyl-LPG as an acyl receptor and oleoyl-CoA over lauroyl-CoA as an acyl donor.
Product Categories/Family for anti-LPGAT1 antibody
References
Yang Y.,et al.J. Biol. Chem. 279:55866-55874 (2004). Ji D.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases. Nagase T.,et al.DNA Res. 3:321-329 (1996). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43089 Da
NCBI Official Full Name
acyl-CoA:lysophosphatidylglycerol acyltransferase 1
NCBI Official Synonym Full Names
lysophosphatidylglycerol acyltransferase 1
NCBI Official Symbol
LPGAT1
NCBI Official Synonym Symbols
NET8; FAM34A; FAM34A1
NCBI Protein Information
acyl-CoA:lysophosphatidylglycerol acyltransferase 1
UniProt Protein Name
Acyl-CoA:lysophosphatidylglycerol acyltransferase 1
UniProt Gene Name
LPGAT1
UniProt Synonym Gene Names
FAM34A; KIAA0205
UniProt Entry Name
LGAT1_HUMAN

NCBI Description

This gene encodes a member of the lysophospholipid acyltransferase family. The encoded protein catalyzes the reacylation of lysophosphatidylglycerol to phosphatidylglycerol, a membrane phospholipid that is an important precursor for the synthesis of cardiolipin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

LPGAT1: Lysophoshatidylglycerol (LPG) specific acyltransferase that recognizes various acyl-CoAs and LPGs as substrates but demonstrates a clear preference for long chain saturated fatty acyl-CoAs and oleoyl-CoA as acyl donors. Prefers oleoyl-LPG over palmitoyl-LPG as an acyl receptor and oleoyl-CoA over lauroyl-CoA as an acyl donor. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Transferase; Membrane protein, multi-pass; EC 2.3.1.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cytoplasm; endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity; 2-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid biosynthetic process

Research Articles on LPGAT1

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Product Notes

The LPGAT1 lpgat1 (Catalog #AAA9217175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LPGAT1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LPGAT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the LPGAT1 lpgat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LPGAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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