Rabbit anti-Human, Mouse PFKM Polyclonal Antibody | anti-PFKM antibody

PFKM Antibody

Cat. Num. AAA7048871

• Gene Names: PFKM; GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: PFKM; Polyclonal Antibody; PFKM Antibody; ATP-dependent 6-phosphofructokinase; muscle typeUniRule annotation; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; PFKX; anti-PFKM antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 780

• Applicable Applications for anti-PFKM antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 1000-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant human ATP-dependent 6-phosphofructokinase, muscle type protein (166-334AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,HEK293 whole cell lysate,Mouse heart tissueAll lanes: PFKM antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 86 kDaObserved band size: 86 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human endometrial cancer at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of A549 cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-PFKM antibody

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1, 6-bisphosphate by ATP, the first committing step of glycolysis.

NCBI and Uniprot Product Information

• NCBI GI #: 4505749
• NCBI GeneID: 5213
• NCBI Accession #: NP_000280.1
• NCBI GenBank Nucleotide #: NM_000289.5
• UniProt Accession #: P08237; Q16814; Q16815; Q6ZTT1; J3KNX3
• Molecular Weight: 93,254 Da
• NCBI Official Full Name: ATP-dependent 6-phosphofructokinase, muscle type isoform 2
• NCBI Official Synonym Full Names: phosphofructokinase, muscle
• NCBI Official Symbol: PFKM
• NCBI Official Synonym Symbols: GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122
• NCBI Protein Information: ATP-dependent 6-phosphofructokinase, muscle type
• UniProt Protein Name: ATP-dependent 6-phosphofructokinase, muscle type
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKM
• UniProt Synonym Gene Names: PFKX; ATP-PFK; PFK-M
• UniProt Entry Name: PFKAM_HUMAN

NCBI Description

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

PFKM: phosphofructokinase, muscle type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Defects in PFKM are the cause of glycogen storage disease VII (GSD-VII) also known as Tarui disease. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Kinase, other

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: 6-phosphofructokinase complex; apical plasma membrane; cytosol

Molecular Function: 6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; protein binding; protein C-terminus binding

Biological Process: fructose 6-phosphate metabolic process; glycolysis; muscle maintenance; protein oligomerization

Disease: Glycogen Storage Disease Vii