Rabbit PFKM Polyclonal Antibody | anti-PFKM antibody

PFKM Antibody

Cat. Num. AAA9403404

• Gene Names: PFKM; GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PFKM; Polyclonal Antibody; PFKM Antibody; GSD7; PFK1; PFKA; PFKX; PFK-1; anti-PFKM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PFKM protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 780

• Applicable Applications for anti-PFKM antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PFKM.
• Target Name: PFKM
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PFKM antibody.)

Related Product Information for anti-PFKM antibody

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-PFKM antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505749
• NCBI GeneID: 5213
• NCBI Accession #: NP_000280.1
• NCBI GenBank Nucleotide #: NM_000289.5
• UniProt Accession #: P08237; Q16814; Q16815; Q6ZTT1; J3KNX3
• Molecular Weight: 93,254 Da
• NCBI Official Full Name: ATP-dependent 6-phosphofructokinase, muscle type isoform 2
• NCBI Official Synonym Full Names: phosphofructokinase, muscle
• NCBI Official Symbol: PFKM
• NCBI Official Synonym Symbols: GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122
• NCBI Protein Information: ATP-dependent 6-phosphofructokinase, muscle type
• UniProt Protein Name: ATP-dependent 6-phosphofructokinase, muscle type
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKM
• UniProt Synonym Gene Names: PFKX; ATP-PFK; PFK-M
• UniProt Entry Name: PFKAM_HUMAN

NCBI Description

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

PFKM: phosphofructokinase, muscle type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Defects in PFKM are the cause of glycogen storage disease VII (GSD-VII) also known as Tarui disease. Two alternatively spliced isoforms have been described.

Protein type: Kinase, other; Carbohydrate Metabolism - galactose; EC 2.7.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - pentose phosphate pathway

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: 6-phosphofructokinase complex; apical plasma membrane; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein homodimerization activity; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity

Biological Process: muscle maintenance; glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; positive regulation of insulin secretion; glucose metabolic process; pathogenesis; glucose homeostasis; fructose 6-phosphate metabolic process; protein oligomerization

Disease: Glycogen Storage Disease Vii