Western Blot (WB)
(Western BlotPositive WB detected in:HepG2 whole cell lysate,HL60 whole cell lysate,Mouse liver tissueAll lanes: PFKL antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 86,91 kDaObserved band size: 86,50,40 kDa)
Rabbit anti-Human, Mouse PFKL Polyclonal Antibody | anti-PFKL antibody
PFKL Antibody
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(Western BlotPositive WB detected in:HepG2 whole cell lysate,HL60 whole cell lysate,Mouse liver tissueAll lanes: PFKL antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 86,91 kDaObserved band size: 86,50,40 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in:HepG2 whole cell lysate,HL60 whole cell lysate,Mouse liver tissueAll lanes: PFKL antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 86,91 kDaObserved band size: 86,50,40 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7048867 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7048867 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunofluorescent analysis of Hela cells using MBS7048867 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC)
(Immunofluorescent analysis of Hela cells using MBS7048867 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Uniprot Description
PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.
Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Kinase, other
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: 6-phosphofructokinase complex; cytosol; membrane
Molecular Function: 6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; protein binding
Biological Process: fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; glycolysis; protein oligomerization; response to glucose stimulus
Research Articles on PFKL
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Product Notes
The PFKL pfkl (Catalog #AAA7048867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKL Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PFKL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 1000-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the PFKL pfkl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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