Rabbit NF-M Polyclonal Antibody | anti-NF-M antibody

Anti-NF-M (Rabbit Polyclonal) Antibody

Cat. Num. AAA416863

• Gene Names: NEFM; NFM; NEF3; NF-M
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Synonyms: NF-M; Polyclonal Antibody; Anti-NF-M (Rabbit Polyclonal) Antibody; Anti-NF-M (Rabbit Polyclonal); anti-NF-M antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: NF-M from human, rat, mouse, cat, chicken.
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 916

• Applicable Applications for anti-NF-M antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Immunofluorescence: 1:250 to 1:500. ABC or other enzyme linked immunocytochemical procedures: 1:2,500 for. Western blots: 1:2,000.
• Immunogen: Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M.
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Testing Data #2

Product Categories/Family for anti-NF-M antibody

Cytoskeletal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 157738649
• NCBI GeneID: 4741
• NCBI Accession #: NP_005373.2
• NCBI GenBank Nucleotide #: NM_005382.2
• UniProt Accession #: P07197; Q4QRK6; B4DGN2; E9PBF7
• Molecular Weight: 102,472 Da
• NCBI Official Full Name: neurofilament medium polypeptide isoform 1
• NCBI Official Synonym Full Names: neurofilament, medium polypeptide
• NCBI Official Symbol: NEFM
• NCBI Official Synonym Symbols: NFM; NEF3; NF-M
• NCBI Protein Information: neurofilament medium polypeptide; neurofilament 3; 160 kDa neurofilament protein; neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide 150kDa
• UniProt Protein Name: Neurofilament medium polypeptide
• UniProt Gene Name: NEFM
• UniProt Synonym Gene Names: NEF3; NFM; NF-M
• UniProt Entry Name: NFM_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Post-translational modification: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Ref.9Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ref.9

Sequence similarities: Belongs to the intermediate filament family.