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Immunofluorescence (IF) (Immunofluorescence staining of HepG2 cells with MBS7107207 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Rabbit anti-Human PFKFB4 Polyclonal Antibody | anti-PFKFB4 antibody

PFKFB4 Antibody

Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
PFKFB4; Polyclonal Antibody; PFKFB4 Antibody; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 4; 6PF-2-K/Fru-2; 6-P2ase 4; PFK/FBPase 4; 6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase; Fructose-2; 6-bisphosphatase; anti-PFKFB4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
498
Applicable Applications for anti-PFKFB4 antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
IF: 1:50-1:200
Immunogen
Recombinant Human 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 protein (266-368AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescence staining of HepG2 cells with MBS7107207 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of HepG2 cells with MBS7107207 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-PFKFB4 antibody
Synthesis and degradation of fructose 2, 6-bisphosphate.
Product Categories/Family for anti-PFKFB4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,203 Da
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform a
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
NCBI Official Symbol
PFKFB4
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
UniProt Gene Name
PFKFB4
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4

NCBI Description

The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Synthesis and degradation of fructose 2,6-bisphosphate.

Research Articles on PFKFB4

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Product Notes

The PFKFB4 pfkfb4 (Catalog #AAA7107207) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKFB4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). IF: 1:50-1:200. Researchers should empirically determine the suitability of the PFKFB4 pfkfb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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