Western Blot (WB)
(Western blot analysis of extracts from 293 cells(heat shock treatment), using PFKFB3 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 53kDa)
Rabbit anti-Human, Rat PFKFB3 Polyclonal Antibody | anti-PFKFB3 antibody
PFKFB3 Antibody
Gene Names
PFKFB3; PFK2; IPFK2
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
PFKFB3; Polyclonal Antibody; PFKFB3 Antibody; 6 phosphofructo 2 kinase/ fructose 2; 6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2; 6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2; 6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2; 6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate; 2 kinase/fructose 2; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK; anti-PFKFB3 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
PFKFB3 Antibody detects endogenous levels of total PFKFB3.
Tissue Specificity: Ubiquitous.
Tissue Specificity: Ubiquitous.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PFKFB3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Application Notes
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human PFKFB3.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation by AMPK stimulates activity.
Similarity
In the C-terminal section; belongs to the phosphoglycerate mutase family.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells(heat shock treatment), using PFKFB3 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 53kDa)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells(heat shock treatment), using PFKFB3 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 53kDa)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6581) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6581) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Related Product Information for anti-PFKFB3 antibody
Synthesis and degradation of fructose 2,6-bisphosphate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,609 Da
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
NCBI Official Symbol
PFKFB3
NCBI Official Synonym Symbols
PFK2; IPFK2
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; iPFK-2; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase 3; renal carcinoma antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofr
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Protein Family
UniProt Gene Name
PFKFB3
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 3
UniProt Entry Name
F263_HUMAN
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Product Notes
The PFKFB3 pfkfb3 (Catalog #AAA9613603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKFB3 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). IF/ICC: 1:100-1:500 WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PFKFB3 pfkfb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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