Western Blot (WB)
(Western blot detection of PFKFB3 in K562 cell lysates using PFKFB3 Rabbit mAb(1:1000 diluted). Predicted band size:60kDa. Observed band size:60kDa.)
Rabbit PFKFB3 Monoclonal Antibody | anti-PFKFB3 antibody
PFKFB3 Rabbit mAb
Gene Names
PFKFB3; PFK2; IPFK2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
PFKFB3; Monoclonal Antibody; PFKFB3 Rabbit mAb; 6-phosphofructo-2-kinase/fructose-2;6-bisphosphatase 3; PFK2; IPFK2; iPFK-2; anti-PFKFB3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R08-7A4
Specificity
Ubiquitous. Gene expression databases
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-PFKFB3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:5000
IHC: 1:20
ICC/IF: 1:20
FC/FACS: 1:20
IP: 1:20
IHC: 1:20
ICC/IF: 1:20
FC/FACS: 1:20
IP: 1:20
Immunogen
Recombinant protein of human PFKFB3
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of PFKFB3 in K562 cell lysates using PFKFB3 Rabbit mAb(1:1000 diluted). Predicted band size:60kDa. Observed band size:60kDa.)
Western Blot (WB)
(Western blot detection of PFKFB3 in K562 cell lysates using PFKFB3 Rabbit mAb(1:1000 diluted). Predicted band size:60kDa. Observed band size:60kDa.)
Related Product Information for anti-PFKFB3 antibody
The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
Synthesis and degradation of fructose 2,6-bisphosphate.
Synthesis and degradation of fructose 2,6-bisphosphate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,609 Da
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
NCBI Official Symbol
PFKFB3
NCBI Official Synonym Symbols
PFK2; IPFK2
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; iPFK-2; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase 3; renal carcinoma antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofr
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Protein Family
UniProt Gene Name
PFKFB3
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 3
UniProt Entry Name
F263_HUMAN
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Product Notes
The PFKFB3 pfkfb3 (Catalog #AAA4752952) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKFB3 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000-1:5000 IHC: 1:20 ICC/IF: 1:20 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the PFKFB3 pfkfb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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