Western Blot (WB)
(Western Blot: Sample: Recombinant NOTCH2, Human.)
Rabbit anti-Human Notch Homolog 2 Polyclonal Antibody | anti-NOTCH2 antibody
Polyclonal Antibody to Notch Homolog 2 (NOTCH2)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-ADWMNRMEVN ETQYNEMFGM VLAPAEGTHP GIAPQSRPPE GKHITTPREP LPPIVTFQLI PKGSIAQPAG APQPQSTCPP AVAGPLPTMY QIPEMARLPS VAFPTAMMPQ QDGQVAQTIL PAYHPFPASV GKYPTPPSQH SYASSNAAER TPSHSGHLQG EHPYLTPSPE SPDQWSSSSP HSASDWSDVT
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant NOTCH2, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NOTCH2, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Uniprot Description
Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.
Chromosomal Location of Human Ortholog: 1p12
Cellular Component: cell surface; endoplasmic reticulum membrane; extracellular region; Golgi membrane; integral component of plasma membrane; membrane; nucleoplasm; nucleus; plasma membrane; receptor complex
Molecular Function: calcium ion binding; protein binding; receptor activity; transcription factor activity, ligand-activated RNA polymerase II transcription factor binding
Biological Process: apoptosis; bone remodeling; cell cycle arrest; cell fate determination; hemopoiesis; intracellular receptor signaling pathway; marginal zone B cell differentiation; multicellular organism development; negative regulation of apoptosis; negative regulation of gene expression; negative regulation of growth rate; nervous system development; Notch signaling pathway; organ morphogenesis; positive regulation of ERK1 and ERK2 cascade; positive regulation of Ras protein signal transduction; pulmonary valve morphogenesis; regulation of developmental process; regulation of transcription, DNA-templated; stem cell maintenance; transcription initiation from RNA polymerase II promoter
Disease: Alagille Syndrome 2; Hajdu-cheney Syndrome
Research Articles on NOTCH2
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Product Notes
The NOTCH2 notch2 (Catalog #AAA2027495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Notch Homolog 2 (NOTCH2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Notch Homolog 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/mL Immunocytochemistry in formalin fixed cells: 5-20ug/mL Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL Immunohistochemistry in paraffin section: 5-20ug/mL Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the NOTCH2 notch2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-ADW MNRMEVN ETQYNEMFGM VLAPAEGTHP GIAPQSRPPE GKHITTPREP LPPIVTFQLI PKGSIAQPAG APQPQSTCPP AVAGPLPTMY QIPEMARLPS VAFPTAMMPQ QDGQVAQTIL PAYHPFPASV GKYPTPPSQH SYASSNAAER TPSHSGHLQG EHPYLTPSPE SPDQWSSSSP HSASDWSDVT. It is sometimes possible for the material contained within the vial of "Notch Homolog 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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