Testing Data
Rabbit anti-Human, Mouse PFKFB3 Polyclonal Antibody | anti-PFKFB3 antibody
PFKFB3 Polyclonal Antibody
Gene Names
PFKFB3; PFK2; IPFK2; iPFK-2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PFKFB3; Polyclonal Antibody; PFKFB3 Polyclonal Antibody; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 3; 6PF-2-K/Fru-2; 6-P2ase 3; PFK/FBPase 3; 6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2; anti-PFKFB3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PFKFB3 polyclonal antibody detects endogenous levels of PFKFB3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
500
Applicable Applications for anti-PFKFB3 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:100
IF: 1:50-1:100
Immunogen
Recombinant full length Human PFKFB3.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Testing Data
Testing Data
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using PFKFB3 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using PFKFB3 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-PFKFB3 antibody
Phosphofructosekinase-2 (PFK-2) belongs to the phosphoglycerate mutase family and is required for the activation of cellular glycolysis. Within the glycolysis pathway, PFK-2 regulates the synthesis and degradation of fructose 2, 6-bisphosphate (F2, 6BP) by enzymatically catalyzing the phosphorylation of fructose-6-phosphate to form F2, 6BP. F2, 6BP functions as a potent activator for 6-phosphofructo-1-kinase that can then activate the glycolysis pathway. Various tissue-specific isoforms of PFK-2 are expressed, including the PFK-2 specific to the brain (br), the liver (liv) and the placenta (pl), and they are also differentially regulated and function as homodimers. A unique isoform, iPFK-2, is induced following proinflammatory stimuli, and it is also constituitively expressed in a variety of carcinoma cell lines, where it leads to an accumation of intracellular F2, 6BP. In addition, the expression of iPFK-2 correlates to increases in DNA sysnthesis, suggesting that iPFK-2 may contribute to cellular transformation of cells and enhanced cellular proliferation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
NCBI Official Symbol
PFKFB3
NCBI Official Synonym Symbols
PFK2; IPFK2; iPFK-2
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Protein Family
UniProt Gene Name
PFKFB3
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 3
UniProt Entry Name
F263_HUMAN
NCBI Description
The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
Research Articles on PFKFB3
Similar Products
Product Notes
The PFKFB3 pfkfb3 (Catalog #AAA3011856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKFB3 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:100. Researchers should empirically determine the suitability of the PFKFB3 pfkfb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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